THE PROCESSING OF ALZHEIMER A4/BETA-AMYLOID PROTEIN-PRECURSOR - IDENTIFICATION OF A HUMAN BRAIN METALLOPEPTIDASE WHICH CLEAVES -LYS-LEU- IN A MODEL PEPTIDE

被引：30

作者：

MCDERMOTT, JR

GIBSON, AM

机构：

[1] MRC Neurochemical Pathology Unit, Newcastle General Hospital, Newcastle upon Tyne, NE4 6BE, Westgate Road

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 179卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)91691-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A search for human brain peptidases with the specificity to cleave the 695 residue A4 β amyloid precursor protein within the -Gln-Lys-Leu- (611-613) sequence was carried out using carbobenzoxy-Gln-Lys-Leu-p-nitroanilide as substrate. A metalloendopeptidase was identified in the soluble fraction of post mortem human cerebral cortex which cleaves the substrate at the Lys-Leu bond. The enzyme was partially purified by anion exchange and size exclusion chromatography; it has a Mr of ∼105-120kda, is inhibited by EDTA but can be reactivated by Mn++ ions, and has maximum activity between pH6.8 and 8. © 1991.

引用

页码：1148 / 1154

页数：7

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