FLUORESCENCE PATTERN-ANALYSIS BASED ON THE TIME-RESOLVED RATIO METHOD

被引：5

作者：

WANG, XF ^{[1
]}

UCHIDA, T ^{[1
]}

MAESHIMA, M ^{[1
]}

MINAMI, S ^{[1
]}

机构：

[1] OSAKA UNIV,FAC ENGN,DEPT APPL PHYS,SUITA,OSAKA 565,JAPAN

来源：

APPLIED SPECTROSCOPY | 1991年 / 45卷 / 04期

关键词：

SPECTROSCOPIC TECHNIQUES; TIME-RESOLVED SPECTROSCOPY; FLUORESCENCE PATTERN ANALYSIS; TIME-RESOLVED RATIO METHOD; GATED IMAGE INTENSIFIER; FLUORESCENCE LIFETIME;

D O I：

10.1366/0003702914336804

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

A new technique is described for component pattern analysis based on time-resolved fluorescence image detection and a ratio method calculation. Differences in fluorescence lifetimes of components are positively utilized to estimate the spatial distribution of each component. Simulation results of the ratio method for fluorescence pattern analysis are discussed. Spatial deconvolution of multicomponent fluorescence data is rapidly carried out from a set of time-resolved, two-dimensional fluorescence data. An example of the application of a multigate time-resolved ratio method for fluorescence pattern analysis is provided. From the results, it is shown that the new method can be successfully used for multicomponent fluorescence pattern analysis at a reasonable signal-to-noise ratio.

引用

页码：560 / 565

页数：6

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