2 POTENTIAL PETUNIA-HYBRIDA MITOCHONDRIAL-DNA REPLICATION ORIGINS SHOW STRUCTURAL AND INVITRO FUNCTIONAL HOMOLOGY WITH THE ANIMAL MITOCHONDRIAL-DNA HEAVY AND LIGHT STRAND REPLICATION ORIGINS

被引：25

作者：

DEHAAS, JM ^{[1
]}

HILLE, J ^{[1
]}

KORS, F ^{[1
]}

VANDERMEER, B ^{[1
]}

KOOL, AJ ^{[1
]}

FOLKERTS, O ^{[1
]}

NIJKAMP, HJJ ^{[1
]}

机构：

[1] CORNELL UNIV, GENET & DEV SECT, ITHACA, NY 14853 USA

来源：

CURRENT GENETICS | 1991年 / 20卷 / 06期

关键词：

MITOCHONDRIAL DNA; PETUNIA-HYBRIDA; PHYSICAL MAP; REPLICATION ORIGIN;

D O I：

10.1007/BF00334779

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Four Petunia hybrida mitochondrial (mt) DNA fragments have been isolated, sequenced, localized on the physical map and analyzed for their ability to initiate specific DNA synthesis. When all four mtDNA fragments were tested as templates in an in vitro DNA synthesizing lysate system, developed from purified P. hybrida mitochondria, specific initiation of DNA synthesis could only be observed starting within two framents, oriA and oriB. When DNA synthesis incubations were performed with DNA templates consisting of both the A and B origins in the same plasmid in complementary strands, DNA synthesis first initiates in the A-origin, proceeds in the direction of the B-origin after which replication is also initiated in the B-origin. Based on these observations, a replication model for the P. hybrida mitochondrial genome is presented.

引用

页码：503 / 513

页数：11

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