FIBROBLAST INTERFERON INDUCES SYNTHESIS OF FOUR PROTEINS IN HUMAN FIBROBLAST CELLS

被引：124
作者：
KNIGHT, E
KORANT, BD
机构：
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 04期
关键词：
D O I：
10.1073/pnas.76.4.1824
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Treatment of human diploid fibroblasts with fibroblast interferon for 8 hr inhibited replication of vesicular stomatitis virus. When the total cell protein of cells treated with interferon for 8 hr was compared to the total cell protein of untreated cells by two-dimensional gel electrophoresis, the interferon-treated cells were found to contain four proteins not found in untreated cells. Addition of actinomycin D to the cells concurrently with interferon inhibited the synthesis of the four proteins. We conclude that these proteins are induced by interferon and that they may be involved in the inhibition of virus replication.
引用
收藏
页码：1824 / 1827
页数：4
相关论文
共 14 条
[1]   SEMI-MICRO, DYE-BINDING ASSAY FOR RABBIT INTERFERON [J].
ARMSTRONG, JA .
APPLIED MICROBIOLOGY, 1971, 21 (04) :723-+
[2]   INCREASED ENDONUCLEASE ACTIVITY IN AN EXTRACT FROM MOUSE EHRLICH ASCITES TUMOR-CELLS WHICH HAD BEEN TREATED WITH A PARTIALLY PURIFIED INTERFERON PREPARATION - DEPENDENCE ON DOUBLE-STRANDED-RNA [J].
BROWN, GE ;
LEBLEU, B ;
KAWAKITA, M ;
SHAILA, S ;
SEN, GC ;
LENGYEL, P .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1976, 69 (01) :114-122
[3]   INCREASED SENSITIVITY OF CELL-FREE PROTEIN-SYNTHESIS TO DOUBLE-STRANDED-RNA AFTER INTERFERON TREATMENT [J].
KERR, IM ;
BROWN, RE ;
BALL, LA .
NATURE, 1974, 250 (5461) :57-59
[4]   PPPA2'P5'A2'P5'-A - INHIBITOR OF PROTEIN-SYNTHESIS SYNTHESIZED WITH AN ENZYME FRACTION FROM INTERFERON-TREATED CELLS [J].
KERR, IM ;
BROWN, RE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (01) :256-260
[5]   INTERFERON - PURIFICATION AND INITIAL CHARACTERIZATION FROM HUMAN DIPLOID CELLS [J].
KNIGHT, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (02) :520-523
[6]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[7]   INTERFERON, DOUBLE-STRANDED-RNA, AND PROTEIN-PHOSPHORYLATION [J].
LEBLEU, B ;
SEN, GC ;
SHAILA, S ;
CABRER, B ;
LENGYEL, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (09) :3107-3111
[8]  
LINDENMANN J, 1957, BRIT J EXP PATHOL, V38, P551
[9]  
LOCKART ROYCE Z., 1964, BIOCHEM BIOPHYS RES COMMUN, V15, P513, DOI 10.1016/0006-291X(64)90496-6
[10]  
OFARRELL PH, 1975, J BIOL CHEM, V250, P4007
12