EXTENSION OF BRANCHING-PROCESSES FROM HYBRIDS OF BRAIN AND CHINESE-HAMSTER OVARY CELLS

被引：16

作者：

KAO, FT ^{[1
]}

FAIK, P ^{[1
]}

PUCK, TT ^{[1
]}

机构：

[1] UNIV COLORADO, MED CTR, DEPT BIOCHEM BIOPHYS & GENET, DENVER, CO 80262 USA

来源：

EXPERIMENTAL CELL RESEARCH | 1979年 / 122卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0014-4827(79)90563-9

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Stable hybrids formed by fusion of auxotrophic mutants of the transformed CHO-Kl cells with cells isolated from biopsies of Chinese hamster and human brains respectively have been established and characterized. All hybrids exhibited an altered response to cyclic AMP derivatives as compared to the previously described Reverse Transformation reaction exhibited by the parental CHO-Kl cell. Most of such cloned hybrids exhibited little response to cAMP derivatives. Occasional clones extended thin branched processes forming a network-like arrangement. Testololactone synergizes the extension of these processes, Colcemid inhibits it, and cycloheximide does not affect the reaction, in parallel with their respective effects on Reverse Transformation. However, cytochalasin B which inhibits Reverse Transformation and butyrate which affects it in a complex fashion have little or no effect on this process extension. Human chromosomal constitutions of positively and negatively reacting hybrids with human brain cells have been partially characterized. © 1979.

引用

页码：83 / 91

页数：9

共 23 条

[1] EXPRESSION OF DIFFERENTIATED FUNCTIONS IN MOUSE NEUROBLASTOMA MEDIATED BY DIBUTYRYL-CYCLIC ADENOSINE MONOPHOSPHATE [J].

FURMANSKI, P ;

SILVERMAN, DJ ;

LUBIN, M .

NATURE, 1971, 233 (5319) :413-+

[2] CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED SYNTHETIC MEDIUM [J].

HAM, RG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1965, 53 (02) :288-&

[3] MORPHOLOGICAL TRANSFORMATION OF CHINESE HAMSTER CELLS BY DIBUTYRYL ADENOSINE CYCLIC 3'-5'-MONOPHOSPHATE AND TESTOSTERONE [J].

HSIE, AW ;

PUCK, TT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1971, 68 (02) :358-+

[4] MAMMALIAN CELL TRANSFORMATIONS IN-VITRO .2. FURTHER CHANGES IN DIFFERENTIATION STATE ACCOMPANYING CONVERSION OF CHINESE HAMSTER CELLS TO FIBROBLASTIC FORM BY DIBUTYRYL ADENOSINE CYCLIC 3',5'-MONOPHOSPHATE AND HORMONES - (CONTACT INHIBITION/COLLAGEN SYNTHESIS/AGGLUTINATION) [J].

HSIE, AW ;

JONES, C ;

PUCK, TT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1971, 68 (07) :1648-&

[5]

Kao F T, 1974, Methods Cell Biol, V8, P23, DOI 10.1016/S0091-679X(08)60441-0

[6] GENETICS OF SOMATIC MAMMALIAN CELLS .10. COMPLEMENTATION ANALYSIS OF GLYCINE-REQUIRING MUTANTS [J].

KAO, FT ;

CHASIN, L ;

PUCK, TT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1969, 64 (04) :1284-+

[7] GENETICS OF SOMATIC MAMMALIAN-CELLS .20. GENETICS OF SOMATIC MAMMALIAN-CELLS - GENETIC, IMMUNOLOGICAL, AND BIOCHEMICAL ANALYSIS WITH CHINESE-HAMSTER CELL HYBRIDS CONTAINING SELECTED HUMAN CHROMOSOMES [J].

KAO, FT ;

JONES, C ;

PUCK, TT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (01) :193-197

[8] COMPLEMENTATION ANALYSIS ON VIRUS-FUSED CHINESE HAMSTER CELLS WITH NUTRITIONAL MARKERS [J].

KAO, FT ;

JOHNSON, RT ;

PUCK, TT .

SCIENCE, 1969, 164 (3877) :313-+

[9] GENETICS OF SOMATIC MAMMALIAN CELLS - LINKAGE STUDIES WITH HUMAN-CHINESE HAMSTER CELL HYBRIDS [J].

KAO, FT ;

PUCK, TT .

NATURE, 1970, 228 (5269) :329-+

[10] GENETICS OF SOMATIC MAMMALIAN CELLS .9. QUANTITATION OF MUTAGENESIS BY PHYSICAL AND CHEMICAL AGENTS [J].

KAO, FT ;

PUCK, TT .

JOURNAL OF CELLULAR PHYSIOLOGY, 1969, 74 (03) :245-+

← 1 2 3 →