SOLID-PHASE RADIOIMMUNOASSAY TECHNIQUES FOR THE DETECTION OF AFRICAN SWINE FEVER ANTIGEN AND ANTIBODY

被引：15
作者：
CROWTHER, JR
WARDLEY, RC
WILKINSON, PJ
机构：
[1] Animal Virus Research Institute, Pirbright, Surrey
来源：
JOURNAL OF HYGIENE | 1979年 / 83卷 / 02期
关键词：
D O I：
10.1017/S0022172400026140
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
A solid phase radioimmunoassay (RIA) has been successfully developed to measure both African swine fever virus (ASFV) antigen and antibody. Studies show that the assay is reproducible and will detect limiting antigen concentrations equivalent to 50–500 HAD50/ml. Both direct and indirect antibody RIA have been developed and have proved to be approximately 100 times more sensitive than the complement fixation test at present available and 1000 times more sensitive than the immuno-electro-osmophoresis test for the detection of ASFV antibody. © 1979, Cambridge University Press
引用
收藏
页码：353 / 361
页数：9
相关论文
共 50 条
  • [31] AFRICAN SWINE FEVER - APPLICATION OF IMMUNOELECTROOSMOPHORESIS FOR DETECTION OF ANTIBODY
    PAN, IC
    DEBOER, CJ
    HESS, WR
    CANADIAN JOURNAL OF COMPARATIVE MEDICINE, 1972, 36 (06): : 309 - &
  • [32] DETECTION OF MONOCLONAL ANTIBODY-DEFINED COLORECTAL-CARCINOMA ANTIGEN BY SOLID-PHASE BINDING-INHIBITION RADIOIMMUNOASSAY
    CHANG, TH
    STEPLEWSKI, Z
    SEARS, HF
    KOPROWSKI, H
    HYBRIDOMA, 1981, 1 (01): : 37 - 45
  • [33] SOLID-PHASE RADIOIMMUNOASSAY FOR THE DETECTION OF ANTIBODIES TO COLLAGEN
    CLAGUE, RB
    BROWN, RA
    WEISS, JB
    LENNOXHOLT, PJ
    JOURNAL OF IMMUNOLOGICAL METHODS, 1979, 27 (01) : 31 - 41
  • [34] SOLID-PHASE RADIOIMMUNOASSAY FOR THE DETECTION OF ANTIBODIES TO COLLAGENS
    CHARRIERE, G
    HARTMANN, DJ
    VILLE, G
    COMPTES RENDUS DES SEANCES DE LA SOCIETE DE BIOLOGIE ET DE SES FILIALES, 1984, 178 (02): : 160 - 170
  • [35] SOLID-PHASE RADIOIMMUNOASSAY FOR THE DETECTION OF ANTIBODIES TO COLLAGENS
    CHARRIERE, G
    HARTMANN, DJ
    VILLE, G
    EUROPEAN JOURNAL OF NUCLEAR MEDICINE, 1984, 9 (07): : A78 - A78
  • [36] A SOLID-PHASE RADIOIMMUNOASSAY FOR DETECTION OF TETANUS ANTIBODIES
    DOW, BC
    BARR, A
    CRAWFORD, RJ
    MITCHELL, R
    MEDICAL LABORATORY SCIENCES, 1983, 40 (01): : 73 - 74
  • [37] SOLID-PHASE RADIOIMMUNOASSAY FOR DETECTION OF ANTIBODIES TO RIBOSOMES
    CAVANAGH, D
    ANALYTICAL BIOCHEMISTRY, 1977, 79 (1-2) : 217 - 225
  • [38] SOLID-PHASE RADIOIMMUNOASSAY FOR QUANTITATION OF HUMAN REAGINIC ANTIBODY AGAINST RAGWEED ANTIGEN-E
    ZEISS, CR
    PRUZANSKY, JJ
    PATTERSON, R
    ROBERTS, M
    JOURNAL OF IMMUNOLOGY, 1973, 110 (02) : 414 - 421
  • [39] ANTIBODY TO HEPATITIS ASSOCIATED ANTIGEN IN BLOOD-DONORS OF DELHI BY SANDWICH SOLID-PHASE RADIOIMMUNOASSAY
    SAMA, SK
    GERA, KL
    GANDHI, PC
    GANDHI, GS
    INDIAN JOURNAL OF MEDICAL RESEARCH, 1973, 61 (09) : 1360 - 1365
  • [40] COMPARATIVE INHIBITORY EFFECTS OF ANTIGEN AND ANTIBODY IN STAPHYLOCOCCAL-ENTEROTOXIN SOLID-PHASE RADIOIMMUNOASSAY SYSTEM
    BUKOVIC, JA
    JOHNSON, HM
    PEELER, JT
    APPLIED MICROBIOLOGY, 1975, 29 (06) : 765 - 768
12345