Evaluation of Antimicrobial Efficacy of Ozonated Sesame Oil, Calcium Hydroxide and their Combination as Intracanal Medicament against Candida Albicans: An in-vitro study

Aim: This in vitro study was done to evaluate the antimicrobial effect of intracanal medicament in root canals contaminated with Candida albicans. Materials and Method: Twenty four extracted human single rooted teeth were selected. Access preparations and biomechanical preparations were done. Specimens were first sterilized and contaminated with Candida and incubated for 48hrs. Confirmation of Candida was done and then divided into 3 experimental groups and 2 control groups. The experimental groups were having 6 teeth each and they were treated with A) Ozonised oil, B) Calcium hydroxide, C) Ozonised oil + Calcium hydroxide respectively. The control groups were further subdivided in 1) Negative control and 2) Positive control with two teeth each. Intracanal medicament was placed into each root canal corresponding to the groups. First sampling was done after 48hrs and second or final sampling was done after one week of placement of intracanal medicament. Microbial growth was checked by counting CFU (Colony forming units). Results: In the first sampling ozonised oil was 100% efficient followed by Calcium hydroxide group while combination of Ozonised oil + Calcium hydroxide showed similar results. In the second or final sampling after one week ozonised oil was highly efficient when compared to other groups. Calcium hydroxide was moderately efficient whereas combination of ozonised oil + calcium hydroxide was least effective as it showed highest CFU/ml. Conclusion: Ozonised oil was most effective for longer duration when compared to other groups and can be used as an alternative intracanal medicament.