PREPARATIVE ENANTIOMER SEPARATION WITH MODIFIED CYCLODEXTRINS AS CHIRAL STATIONARY PHASES

Preparative gas chromatographic enantiomer separation was achieved by using a packed column with heptakis(2,6-di-O-methyl-3-O-pentyl)-beta-cyclodextrin (2,6-Me-3-Pe-beta-CD) as a chiral stationary phase. Depending on the selectivity factor up to milligram amounts of almost pure enantiomers or at least enantiomeric mixtures, sufficiently enriched to determine the sign of optical rotation, could be obtained with only one injection of a racemate. This technique may be very useful for stereochemical assignments, for attaining pure enantiomers for bioassays, pharmacokinetic and metabolic studies and investigations of fragrance and flavour compounds.