ENHANCED CELLULASE PRODUCTION BY A MUTANT OF TRICHODERMA-REESEI

被引：64

作者：

GADGIL, NJ ^{[1
]}

DAGINAWALA, HF ^{[1
]}

CHAKRABARTI, T ^{[1
]}

KHANNA, P ^{[1
]}

机构：

[1] NAGPUR UNIV, DEPT BIOCHEM, NAGPUR, MAHARASHTRA, INDIA

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1995年 / 17卷 / 10期

关键词：

LIGNOCELLULOSICS; TRICHODERMA REESEI QM9414; HYPERPRODUCING MUTANT; FEEDBACK INHIBITION; ENZYME PURIFICATION;

D O I：

10.1016/0141-0229(94)00131-A

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The need for renewable energy resources has focused attention on the renewable polysaccharide cellulose, which can be enzymatically hydrolyzed to yield sugars. Trichoderma reesei QM9414, a cellulolytic microorganism, was subjected to mutagenesis using a combination of ultraviolet light and sodium nitrite (45 min, 0.5 mg ml(-1)). A cellulase hyperproducing mutant, whose enzyme is more resistant to catabolite repression by glucose and cellobiose, was isolated. Enhancement in cellulase yields (1.5- to 1.75-fold) and hydrolytic potential (1.2-fold) was achieved. The mild chemical mutagen used in this research has not been reported before.

引用

页码：942 / 946

页数：5

共 20 条

[1] EFFECTS OF STRAIN AND FERMENTATION CONDITIONS ON PRODUCTION OF CELLULASE BY TRICHODERMA-REESEI [J].