DETECTION OF A POINT MUTATION IN CHOLESTERYL ESTER TRANSFER PROTEIN GENE BY POLYMERASE CHAIN REACTION-MEDIATED SITE-DIRECTED MUTAGENESIS

被引：8
|
作者：
MATSUNAGA, A [1 ]
ARAKI, K [1 ]
MORIYAMA, K [1 ]
HANDA, K [1 ]
ARAKAWA, F [1 ]
NISHI, K [1 ]
SASAKI, J [1 ]
ARAKAWA, K [1 ]
机构：
[1] FUKUOKA UNIV, SCH MED, DEPT BIOCHEM, FUKUOKA 81401, JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1166卷 / 01期
关键词：
CHOLESTERYL ESTER TRANSFER PROTEIN; PCR-MEDIATED SITE-DIRECTED MUTAGENESIS;
D O I：
10.1016/0005-2760(93)90293-I
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe a method for the rapid and non-radioactive examination of DNA samples for a mutation of cholesteryl ester transfer protein using a polymerase chain reaction-mediated site-directed mutagenesis. CETP deficiencies were studied in 554 Japanese subjects (370 men, 184 women) aged between 18 and 91 (mean 48.3 years). By this method, we detected one homozygote and 3 heterozygotes of the CETP deficiency.
引用
收藏
页码：131 / 134
页数：4
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