DETECTION OF A POINT MUTATION IN CHOLESTERYL ESTER TRANSFER PROTEIN GENE BY POLYMERASE CHAIN REACTION-MEDIATED SITE-DIRECTED MUTAGENESIS

被引：8
|
作者：
MATSUNAGA, A [1 ]
ARAKI, K [1 ]
MORIYAMA, K [1 ]
HANDA, K [1 ]
ARAKAWA, F [1 ]
NISHI, K [1 ]
SASAKI, J [1 ]
ARAKAWA, K [1 ]
机构：
[1] FUKUOKA UNIV, SCH MED, DEPT BIOCHEM, FUKUOKA 81401, JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1166卷 / 01期
关键词：
CHOLESTERYL ESTER TRANSFER PROTEIN; PCR-MEDIATED SITE-DIRECTED MUTAGENESIS;
D O I：
10.1016/0005-2760(93)90293-I
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe a method for the rapid and non-radioactive examination of DNA samples for a mutation of cholesteryl ester transfer protein using a polymerase chain reaction-mediated site-directed mutagenesis. CETP deficiencies were studied in 554 Japanese subjects (370 men, 184 women) aged between 18 and 91 (mean 48.3 years). By this method, we detected one homozygote and 3 heterozygotes of the CETP deficiency.
引用
收藏
页码：131 / 134
页数：4
相关论文
共 50 条
  • [21] APPROACH TO IDENTIFICATION OF A POINT MUTATION IN APO B100 GENE BY MEANS OF A PCR-MEDIATED SITE-DIRECTED MUTAGENESIS
    SCHWARTZ, EI
    SHEVTSOV, SP
    KUCHINSKI, AP
    KOVALEV, YP
    PLUTALOV, OV
    BERLIN, YA
    NUCLEIC ACIDS RESEARCH, 1991, 19 (13) : 3752 - 3752
  • [22] HIGH-YIELD METHOD FOR SITE-DIRECTED MUTAGENESIS USING POLYMERASE CHAIN-REACTION AND 3 PRIMERS
    STEINBERG, RA
    GORMAN, KB
    ANALYTICAL BIOCHEMISTRY, 1994, 219 (01) : 155 - 157
  • [23] SITE-DIRECTED, RECOMBINATION-MEDIATED MUTAGENESIS OF A COMPLEX GENE LOCUS
    BARTON, MC
    HOEKSTRA, MF
    EMERSON, BM
    NUCLEIC ACIDS RESEARCH, 1990, 18 (24) : 7349 - 7355
  • [24] DETECTION OF 12 CYSTIC-FIBROSIS MUTATIONS USING POLYMERASE CHAIN-REACTION MEDIATED SITE DIRECTED MUTAGENESIS (PSM)
    FRIEDMAN, KJ
    SILVERMAN, LM
    PERRY, TR
    HIGHSMITH, WE
    AMERICAN JOURNAL OF HUMAN GENETICS, 1991, 49 (04) : 187 - 187
  • [25] A common mutation of cholesteryl ester transfer protein gene in dialysis patients
    Kimura, H
    Gejyo, F
    Suzuki, S
    Yamaguchi, T
    Imura, T
    Miyazaki, R
    Ei, K
    Ei, I
    Oda, M
    Miyakawa, Y
    Arakawa, M
    KIDNEY INTERNATIONAL, 1999, 56 : S186 - S189
  • [26] IDENTIFICATION OF AN IMMUNODOMINANT REGION ON THE I-A-BETA CHAIN USING SITE-DIRECTED MUTAGENESIS AND DNA-MEDIATED GENE-TRANSFER
    BUERSTEDDE, JM
    PEASE, LR
    BELL, MP
    NILSON, AE
    BUERSTEDDE, G
    MURPHY, D
    MCKEAN, DJ
    JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 167 (02): : 473 - 487
  • [27] Thermostability enhancement of Escherichia coli phytase by error-prone polymerase chain reaction (epPCR) and site-directed mutagenesis
    Xing, Hongguan
    Wang, Pingping
    Yan, Xing
    Yang, Yi
    Li, Xinliang
    Liu, Rui
    Zhou, Zhihua
    FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2023, 11
  • [28] Protein engineering of γ-Echistatin by site-directed mutagenesis using synthetic gene method
    Wu, SH
    Li, DS
    Chiou, SH
    BIOPHYSICAL JOURNAL, 2000, 78 (01) : 415A - 415A
  • [29] Phylogenetic utility of LEAFY gene in Cinnamomum (Lauraceae): Gene duplication and polymerase chain reaction-mediated recombination
    Huang, Jian-Feng
    Li, Lang
    Conran, John G.
    Li, Jie
    JOURNAL OF SYSTEMATICS AND EVOLUTION, 2016, 54 (03) : 238 - 249
  • [30] CODON-BASED COMBINATORIAL ALANINE SCANNING SITE-DIRECTED MUTAGENESIS - DESIGN, IMPLEMENTATION, AND POLYMERASE CHAIN-REACTION SCREENING
    CHATELLIER, J
    MAZZA, A
    BROUSSEAU, R
    VERNET, T
    ANALYTICAL BIOCHEMISTRY, 1995, 229 (02) : 282 - 290
12345