CONSTRUCTION OF A MOUSE YEAST ARTIFICIAL CHROMOSOME LIBRARY IN A RECOMBINATION-DEFICIENT STRAIN OF YEAST

被引：78

作者：

CHARTIER, FL ^{[1
]}

KEER, JT ^{[1
]}

SUTCLIFFE, MJ ^{[1
]}

HENRIQUES, DA ^{[1
]}

MILEHAM, P ^{[1
]}

BROWN, SDM ^{[1
]}

机构：

[1] ST MARYS HOSP,SCH MED,DEPT BIOCHEM & MOLEC GENET,NORFOLK PL,LONDON W2 1PG,ENGLAND

来源：

NATURE GENETICS | 1992年 / 1卷 / 02期

关键词：

D O I：

10.1038/ng0592-132

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have constructed a new generation yeast artificial chromosome (YAC) library from female C57BL/10 mice in a recombination-deficient strain of Saccharomyces cerevisiae carrying a mutation in the RAD52 gene. The YAC library contains 41,568 clones with an average insert size of 240 kilobases, representing a greater than threefold coverage of the mouse genome. Currently, the library can be screened by polymerase chain reaction and we have isolated positive clones at a number of loci in the mouse genome. This rad52 library should enable a long-term assessment of the effect of one of the yeast recombination pathway genes on both, genome-wide YAC clone stability and the frequency of chimaeric clones.

引用

页码：132 / 136

页数：5

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