The influence of the variable electromagnetic field on the biofilm formed by Candida albicans

The aim of the study was to investigate the impact of variable electromagnetic field of various intensities on the mature structure of the biofilm formed by the yeast Candida albicans. The yeast was grown on YPD medium on Petri dishes, in an incubator at 28 degrees C for 48 hours. Liquid cultures of Candida albicans were grown on YNB medium with the addition of 2% glucose in Erlenmeyer flasks, on a shaker at 28 degrees C at 180 rpm. In order to create a homogeneous electromagnetic field, a solenoid with a length of 48 cm and a diameter of 6 cm was used. The experiment consisted of subjecting the mature biofilm to the action of an electromagnetic field of variable intensity, and thereby, variable magnetic induction values of 20 mT, 40 mT, 70 mT and 90 mT, respectively. The research shown that in most cases the electromagnetic field does not significantly effect on a mature biofilm. The highest optical density values were observed at 20 mT and five and a half and six exposure hours (successively 0.248 MF and 0.166 MF).