SEQUENTIAL INITIATION OF LAGGING AND LEADING STRAND SYNTHESIS BY 2 DIFFERENT POLYMERASE COMPLEXES AT THE SV40 DNA-REPLICATION ORIGIN

被引：387
作者：
TSURIMOTO, T
MELENDY, T
STILLMAN, B
机构：
[1] Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724
来源：
NATURE | 1990年 / 346卷 / 6284期
关键词：
D O I：
10.1038/346534a0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Enzymatic synthesis of DNA from the simian virus 40 origin of DNA replication has been reconstituted in vitro with eight purified components. DNA polymerase α-primase complex first initiates DNA synthesis at the replication origin and continues as the lagging strand polymerase. Subsequently, the DNA polymerase δ complex initiates replication on the leading strand template. Some prokaryotic DNA polymerase complexes can replace the eukaryotic polymerase δ complex. A model for polymerase switching during initiation of DNA replication is presented. © 1990 Nature Publishing Group.
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页码：534 / 539
页数：6
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