COUP-TF GENE - A STRUCTURE UNIQUE FOR THE STEROID THYROID RECEPTOR SUPERFAMILY

被引：62

作者：

RITCHIE, HH ^{[1
]}

WANG, LH ^{[1
]}

TSAI, S ^{[1
]}

OMALLEY, BW ^{[1
]}

TSAI, MJ ^{[1
]}

机构：

[1] BAYLOR UNIV,DEPT CELL BIOL,HOUSTON,TX 77030

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 23期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1093/nar/18.23.6857

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two different genomic genes for the COUP-transcription factor, COUP-TF II and COUP-TF, have been isolated from a human cosmid genomic library using a [P-32]-labeled cDNA probe. Data obtained from Southern blot analysis of these cosmid clones indicated that two closely related genes exist in the human genome and have a similar genomic organization. The genes are similar in the hormone and DNa binding domains but diverge from one another in the N-terminal region. Using DNA sequencing and polymerase chain reaction (PCR) techniques we have determined that the structure of COUP-TF I consists only of three exons and two introns. Surprisingly, both zinc fingers (i.e., F1 and F2) are located in the first exon. Therefore, COUP-TF 1 is unique among the members of the steroid/thyroid hormone receptor superfamily which have been described to date.

引用

页码：6857 / 6862

页数：6

共 28 条

[1] PURIFICATION AND CHARACTERIZATION OF CHICKEN OVALBUMIN GENE UPSTREAM PROMOTER TRANSCRIPTION FACTOR FROM HOMOLOGOUS OVIDUCT CELLS [J].