NUCLEOTIDE-SEQUENCE OF THE TRANSFORMING HINDIII-G FRAGMENT OF ADENOVIRUS TYPE-5 DNA - REGION BETWEEN MAP POSITIONS 4.5 (HPAI SITE) AND 8.0 (HINDIII SITE)

被引：61
作者：
MAAT, J
VANORMONDT, H
机构：
[1] Laboratory for Physiological Chemistry, Sylvius Laboratories, 2333 AL Leiden
来源：
GENE | 1979年 / 6卷 / 01期
关键词：
cell transformation; chain terminators; coding capacity; DNA sequence; Maxam-Gilbert technique; RNA splicing;
D O I：
10.1016/0378-1119(79)90086-6
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The nucleotide sequence of the region between map position 4.5 (HpaI-site) and 8.0 (HindIII-site) of adenovirus type 5 (Ad5) DNA has been determined. This stretch of DNA is part of the transforming HindIII-G fragment, which is 2809 nucleotides long. The sequenced segment was found to have a long open reading frame for protein biosynthesis, starting 23 nucleotides from the HpaI site and extending all the way to the HindIII-G site, which could code for a protein of at least 44 000 daltons. The possible correlation between the coding capacity of the HindIII-G fragment and the transforming" proteins specified by it will be discussed in the light of the recent data on the splicing of early mRNAs. © 1979."
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页码：75 / 90
页数：16
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