ISOLATION OF A HIGHLY ENANTIOSELECTIVE EPOXIDE HYDROLASE FROM RHODOCOCCUS SP NCIMB-11216

被引：42

作者：

MISCHITZ, M ^{[1
]}

FABER, K ^{[1
]}

WILLETTS, A ^{[1
]}

机构：

[1] UNIV EXETER,DEPT BIOL SCI,EXETER EX4 4QG,DEVON,ENGLAND

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 09期

关键词：

D O I：

10.1007/BF00127422

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Whole cells of Rhodococcus sp. NCIMB 11216 catalyze the asymmetric hydrolysis of racemic epoxides giving access to chiral epoxides and diols, which are important chiral building blocks for the, synthesis of bioactive compounds. Employing a four-step purification procedure, the epoxide hydrolase responsible for the reaction was isolated and characterized to be a cofactor-independent, soluble monomeric protein of similar to 35kDa, exhibiting an isoelectric point of 4.7.

引用

页码：893 / 898

页数：6

共 16 条

[1]

ALLEN RH, 1969, J BIOL CHEM, V244, P2078

[2]

BERTI G, 1986, NATO ASI SER C-MATH, V178, P349

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] BIOFORMATION OF OPTICALLY PURE EPOXIDES [J].

DEBONT, JAM .

TETRAHEDRON-ASYMMETRY, 1993, 4 (06) :1331-1340

[5]

Furuhashi K, 1992, CHIRALITY IND, P167

[6] ASYMMETRIC HYDROLYSIS OF EPOXIDES USING AN IMMOBILIZED ENZYME PREPARATION FROM RHODOCOCCUS SP [J].

HECHTBERGER, P ;

WIRNSBERGER, G ;

MISCHITZ, M ;

KLEMPIER, N ;

FABER, K .

TETRAHEDRON-ASYMMETRY, 1993, 4 (06) :1161-1164

[7] CHARACTERIZATION OF THE EPOXIDE HYDROLASE FROM AN EPICHLOROHYDRIN-DEGRADING PSEUDOMONAS-SP [J].

JACOBS, MHJ ;

VANDENWIJNGAARD, AJ ;

PENTENGA, M ;

JANSSEN, DB .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 202 (03) :1217-1222

[8] HIGHLY ENANTIOSELECTIVE EPOXIDATION CATALYSTS DERIVED FROM 1,2-DIAMINOCYCLOHEXANE [J].

JACOBSEN, EN ;

ZHANG, W ;

MUCI, AR ;

ECKER, JR ;

DENG, L .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (18) :7063-7064

[9] ASYMMETRIC EPOXIDATION OF OLEFINS CATALYZED BY MANGANESE COMPLEXES OF CHIRAL STRAPPED PORPHYRINS WITH DIASTEREOTOPIC FACES - A NOVEL STRATEGY FOR STEREOCHEMICAL MODELING OF THE ACTIVE-SITE OF CYTOCHROME-P-450 [J].

KONISHI, K ;

ODA, K ;

NISHIDA, K ;

AIDA, T ;

INOUE, S .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (04) :1313-1317

[10] THE MICROBIAL-PRODUCTION OF EPOXIDES [J].

LEAK, DJ ;

AIKENS, PJ ;

SEYEDMAHMOUDIAN, M .

TRENDS IN BIOTECHNOLOGY, 1992, 10 (07) :256-261

← 1 2 →