HIGH-SENSITIVITY TO INTERNAL TETRAETHYLAMMONIUM IN K(CA) CHANNELS OF CEREBROVASCULAR SMOOTH-MUSCLE CELLS

被引：7

作者：

WANG, YH ^{[1
]}

MATHERS, DA ^{[1
]}

机构：

[1] UNIV BRITISH COLUMBIA,FAC MED,DEPT PHYSIOL,VANCOUVER V6T 1W5,BC,CANADA

来源：

NEUROSCIENCE LETTERS | 1991年 / 132卷 / 02期

基金：

英国医学研究理事会;

关键词：

CEREBRAL ARTERY; SMOOTH MUSCLE; POTASSIUM CHANNEL; CALCIUM; PATCH CLAMP; TETRAETHYLAMMONIUM;

D O I：

10.1016/0304-3940(91)90306-E

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Patch clamp methods were used to study the properties of calcium-dependent K(Ca) channels in enzyme dissociated smooth muscle cells from the cerebral arteries of adult rats. Dissociated muscle cells were maintained at 4-degrees-C for up to 48 h prior to use. Inside-out membrane patches excised from these cells contained a K(Ca) channel with a conductance of 92 +/- 2.6 pS in symmetrical 140 mM potassium solutions. This channel was activated by membrane depolarization and by cytoplasmic calcium, and showed negligible permeability to sodium or cesium ions. Single channel currents were reduced by internal application of tetraethylammonium ions, with a K(d) = 0.31 mM.

引用

页码：222 / 224

页数：3

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