EFFECT OF POLOXAMER-407 ON THE ADHERENCE OF PSEUDOMONAS-AERUGINOSA TO CORNEAL EPITHELIAL-CELLS

被引：0

作者：

PORTOLES, M ^{[1
]}

AUSTIN, F ^{[1
]}

NOSBARBERA, S ^{[1
]}

PATERSON, C ^{[1
]}

REFOJO, MF ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,SCHEPENS EYE RES INST,BOSTON,MA 02114

来源：

CORNEA | 1995年 / 14卷 / 01期

关键词：

BACTERIAL ADHERENCE; ANTIADHESIVES; POLOXAMERS; CORNEAL EPITHELIAL CELLS; PSEUDOMONAS AERUGINOSA;

D O I：

暂无

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

The effect of poloxamer 407 on Pseudomonas aeruginosa adherence to cultured epithelial cells from rabbit corneas was investigated. Three methods of bacterial quantification were used to assess P. aeruginosa adherence: scanning electron microscopy (SEM) counts, radioactivity counts, and viable bacteria counts. Confluent monolayers of rabbit corneal epithelial cells were incubated in agitation for 30 min at room temperature with H-3-labeled or nonradiolabeled P. aeruginosa (10(10) bacteria/ml) in a solution of poloxamer 407 [2% or 4% in phosphate-buffered saline (PBS)] or PBS as control. Cell monolayers were washed to remove nonadherent bacteria and fixed with 2.5% glutaraldehyde and processed for SEM or processed for radioactivity counting or for culture on agar plates. The results showed that both solutions of poloxamer 407 inhibited similar to 75% of the bacterial adherence to epithelial cells (p < 0.05). Similar percentages of bacterial inhibition were obtained using the three methods of bacterial quantification. The use of an antiadherent agent such as poloxamer 407 in eye drops could possibly be a prophylactic approach to P. aeruginosa keratitis.

引用

页码：56 / 61

页数：6