FLUOROMETRIC-DETERMINATION OF THE KINETICS OF ANTHRACYCLINES UPTAKE BY CELLS

被引：5
作者：
ANDREONI, A
COLASANTI, A
KISSLINGER, A
MASTROCINQUE, M
RICCIO, P
ROBERTI, G
机构：
[1] UNIV NAPLES,DIPARTIMENTO BIOL & PATOL CELLULARE & MOLEC,I-80131 NAPLES,ITALY
[2] CNR,CTR ENDOCRINOL & ONCOL SPERIMENTALE,NAPLES,ITALY
[3] UNIV MILAN,DIPARTIMENTO FIS,COMO,ITALY
[4] UNIV NAPLES,DIPARTIMENTO SCI FISICHE,I-80125 NAPLES,ITALY
来源：
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1994年 / 28卷 / 01期
关键词：
ANTHRACYCLINE; DAUNORUBICIN; DOXORUBICIN; FRTL5; CELL; MPTK-6; CELLULAR UPTAKE; FLUORESCENCE;
D O I：
10.1016/0165-022X(94)90064-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Fluorometric measurements on extracellular medium are shown to allow kinetic parameters of in vitro anthracycline uptake by cells to be calculated. The method provides influx and efflux rates, as well as the time dependence of both influx and efflux. It is applied to a normal thyroid epithelial cell line (FRTL-5) and a cell line (MPTK-6) derived from the lung metastases of a thyroid carcinoma exposed to daunorubicin at concentrations within the range of 250 to 1000 ng/ml. The results show that the number of cells influences the dependence of the kinetics upon the extracellular drug concentration and that the MPTK-6 cells are endowed with very efficient efflux mechanisms.
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收藏
页码：53 / 68
页数：16
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