HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE SEPARATION OF CHLORAMBUCIL AND ITS N-OXIDE PRODRUG

被引：7
|
作者：
CHANDLER, KJ [1 ]
MCCABE, JB [1 ]
KIRKPATRICK, DL [1 ]
机构：
[1] UNIV REGINA,DEPT CHEM,REGINA S4S 0A2,SASKATCHEWAN,CANADA
来源：
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 652卷 / 02期
关键词：
D O I：
10.1016/0378-4347(93)E0389-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A reversed-phase high-performance liquid chromatographic method is described to distinguish chlorambucil N-oxide from the parent chlorambucil and quantitate both after separation from biological samples. The influence of solvent pH, alcohol, acid and ion-pairing agent on the separation is described. The stability of chlorambucil and its N-oxide in buffers and alcohols, as well as stability during filtration is discussed with potential application for metabolic studies.
引用
收藏
页码：195 / 202
页数：8
相关论文
共 50 条
  • [1] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF ROSOXACIN AND ITS N-OXIDE METABOLITE IN PLASMA AND URINE
    KULLBERG, MP
    KOSS, R
    ONEIL, S
    EDELSON, J
    JOURNAL OF CHROMATOGRAPHY, 1979, 173 (01): : 155 - 163
  • [2] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR A CLOZAPINE ANALOG, CGS-13429, AND ITS N-OXIDE AND DESMETHYL METABOLITES
    CHOVAN, JP
    VERMEULEN, JD
    JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 494 : 413 - 419
  • [3] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR SEPARATION OF DINUCLEOTIDES
    HAGEMEIER, E
    BORNEMANN, S
    BOOS, KS
    SCHLIMME, E
    JOURNAL OF CHROMATOGRAPHY, 1982, 237 (01): : 174 - 177
  • [4] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY OF AMPICILLIN AND ITS PRODRUG LENAMPICILLIN
    MARZO, A
    MONTI, N
    RIPAMONTI, M
    MARTELLI, EA
    PICARI, M
    JOURNAL OF CHROMATOGRAPHY, 1990, 507 : 235 - 239
  • [5] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF FENTHION AND ITS METABOLITES
    CABRAS, P
    PLUMITALLO, A
    SPANEDDA, L
    JOURNAL OF CHROMATOGRAPHY, 1991, 540 (1-2): : 406 - 410
  • [6] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC MEASUREMENT OF NICOTINAMIDE N-OXIDE IN URINE AFTER EXTRACTING WITH CHLOROFORM
    SHIBATA, K
    AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1989, 53 (05): : 1329 - 1331
  • [7] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF COBALAMINS
    FRENKEL, EP
    KITCHENS, RL
    PROUGH, R
    JOURNAL OF CHROMATOGRAPHY, 1979, 174 (02): : 393 - 400
  • [8] High-performance liquid chromatographic determination of propiverine and its N-oxide in human serum
    Richter, K
    Scheithauer, S
    Thümmler, D
    JOURNAL OF CHROMATOGRAPHY B, 1998, 708 (1-2): : 325 - 329
  • [9] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF CYROMAZINE AND ITS METABOLITE MELAMINE
    CABRAS, P
    MELONI, M
    SPANEDDA, L
    JOURNAL OF CHROMATOGRAPHY, 1990, 505 (02): : 413 - 416
  • [10] ISOCRATIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE SEPARATION OF TESTOSTERONE METABOLITES
    SANWALD, P
    BLANKSON, EA
    DULERY, BD
    SCHOUN, J
    HUEBERT, ND
    DOW, J
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1995, 672 (02): : 207 - 215
12345