A PROTEIN OF THE ENDOPLASMIC-RETICULUM INVOLVED EARLY IN POLYPEPTIDE TRANSLOCATION

被引：264
作者：
GORLICH, D
HARTMANN, E
PREHN, S
RAPOPORT, TA
机构：
[1] MAX DELBRUCK CTR MOLEC MED, ROBERT ROSSLE STR 10, O-1115 BERLIN, GERMANY
[2] HUMBOLDT UNIV, INST BIOCHEM, O-1040 BERLIN, GERMANY
来源：
NATURE | 1992年 / 357卷 / 6373期
关键词：
D O I：
10.1038/357047a0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
To identify components of the mammalian endoplasmic reticulum involved in the translocation of secretory proteins, crosslinking and reconstitution methods were combined. A multispanning abundant membrane glycoprotein was found which is in proximity to nascent chains early in translocation. In reconstituted proteoliposomes, this protein is stimulatory or required for the translocation of secretory proteins.
引用
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页码：47 / 52
页数：6
相关论文
共 31 条
[1]   A YEAST MITOCHONDRIAL OUTER-MEMBRANE PROTEIN ESSENTIAL FOR PROTEIN IMPORT AND CELL VIABILITY [J].
BAKER, KP ;
SCHANIEL, A ;
VESTWEBER, D ;
SCHATZ, G .
NATURE, 1990, 348 (6302) :605-609
[2]   THE SIGNAL RECOGNITION PARTICLE RECEPTOR MEDIATES THE GTP-DEPENDENT DISPLACEMENT OF SRP FROM THE SIGNAL SEQUENCE OF THE NASCENT POLYPEPTIDE [J].
CONNOLLY, T ;
GILMORE, R .
CELL, 1989, 57 (04) :599-610
[3]   ASSEMBLY OF YEAST SEC PROTEINS INVOLVED IN TRANSLOCATION INTO THE ENDOPLASMIC-RETICULUM INTO A MEMBRANE-BOUND MULTISUBUNIT COMPLEX [J].
DESHAIES, RJ ;
SANDERS, SL ;
FELDHEIM, DA ;
SCHEKMAN, R .
NATURE, 1991, 349 (6312) :806-808
[4]   A YEAST MUTANT DEFECTIVE AT AN EARLY STAGE IN IMPORT OF SECRETORY PROTEIN PRECURSORS INTO THE ENDOPLASMIC-RETICULUM [J].
DESHAIES, RJ ;
SCHEKMAN, R .
JOURNAL OF CELL BIOLOGY, 1987, 105 (02) :633-645
[5]   PURIFICATION OF MICROSOMAL SIGNAL PEPTIDASE AS A COMPLEX [J].
EVANS, EA ;
GILMORE, R ;
BLOBEL, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (03) :581-585
[6]   PROTEIN TRANSLOCATION ACROSS THE ENDOPLASMIC-RETICULUM .2. ISOLATION AND CHARACTERIZATION OF THE SIGNAL RECOGNITION PARTICLE RECEPTOR [J].
GILMORE, R ;
WALTER, P ;
BLOBEL, G .
JOURNAL OF CELL BIOLOGY, 1982, 95 (02) :470-477
[7]   THE SIGNAL SEQUENCE RECEPTOR HAS A 2ND SUBUNIT AND IS PART OF A TRANSLOCATION COMPLEX IN THE ENDOPLASMIC-RETICULUM AS PROBED BY BIFUNCTIONAL REAGENTS [J].
GORLICH, D ;
PREHN, S ;
HARTMANN, E ;
HERZ, J ;
OTTO, A ;
KRAFT, R ;
WIEDMANN, M ;
KNESPEL, S ;
DOBBERSTEIN, B ;
RAPOPORT, TA .
JOURNAL OF CELL BIOLOGY, 1990, 111 (06) :2283-2294
[8]   MUTANTS IN 3 NOVEL COMPLEMENTATION GROUPS INHIBIT MEMBRANE-PROTEIN INSERTION INTO AND SOLUBLE-PROTEIN TRANSLOCATION ACROSS THE ENDOPLASMIC-RETICULUM MEMBRANE OF SACCHAROMYCES-CEREVISIAE [J].
GREEN, N ;
FANG, H ;
WALTER, P .
JOURNAL OF CELL BIOLOGY, 1992, 116 (03) :597-604
[9]   THE SIGNAL RECOGNITION PARTICLE IN SACCHAROMYCES-CEREVISIAE [J].
HANN, BC ;
WALTER, P .
CELL, 1991, 67 (01) :131-144
[10]   A MEMBRANE COMPONENT OF THE ENDOPLASMIC-RETICULUM THAT MAY BE ESSENTIAL FOR PROTEIN TRANSLOCATION [J].
HARTMANN, E ;
WIEDMANN, M ;
RAPOPORT, TA .
EMBO JOURNAL, 1989, 8 (08) :2225-2229
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