Applications of Molecularly Imprinted Column for Chiral Separation by High Performance Liquid Chromatography and Capillary Electrochromatography

被引：0

作者：

Ou Junjie ^{[1
]}

Dong Jing ^{[1
]}

Wu Minghuo ^{[1
]}

Kong Liang ^{[1
]}

Zou Hanfa ^{[1
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

来源：

CHINESE JOURNAL OF CHROMATOGRAPHY | 2007年 / 25卷 / 02期

关键词：

molecular imprinting; monolithic column; high performance liquid chromatography (HPLC); capillary electrochromatography (CEC); chiral separation; Troger' s base; 1,1'-bi-2-naphthol;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The molecularly imprinted polymer (MIP) monolithic column was firstly in situ polymerized in a traditional stainless-steel chromatographic column by using methylacrylic acid and S-Troger's base as the functional monomer and imprinted template molecules, respectively. The effects of the content of acetic acid and water in mobile phase on the separation were systematically investigated. The fast enantioseparation of the racemate on monolithic column was successfully realized by stepwise gradient elution. The MIP monolithic capillary column was also synthesized in a fused-silica capillary by in situ polymerization with 2-(dimethylamino) ethyl methacrylate (DAMA) as functional monomer. The enantioseparation of 1,1'-bi-2-naphthol (BNL) on the obtained MIP capillary column was achieved in capillary electrochromatography (CEC). The results indicated that other acidic template can be imprinted by using basic DAMA as functional monomer, which enlarge the application fields of imprinting molecules.

引用

页码：129 / 134

页数：6

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