MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY OF ENZYMATICALLY SYNTHESIZED RNA UP TO 150 KDA

被引：93
作者：
KIRPEKAR, F
NORDHOFF, E
KRISTIANSEN, K
ROEPSTORFF, P
LEZIUS, A
HAHNER, S
KARAS, M
HILLENKAMP, F
机构：
[1] UNIV MUNSTER,INST MED PHYS & BIOPHYS,D-48149 MUNSTER,GERMANY
[2] UNIV MUNSTER,INST BIOCHEM,D-48149 MUNSTER,GERMANY
来源：
NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 19期
关键词：
D O I：
10.1093/nar/22.19.3866
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.
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页码：3866 / 3870
页数：5
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