CLONING OF A NOVEL RAT-KIDNEY CDNA HOMOLOGOUS TO CHIP28 AND WCH-CD WATER CHANNELS

被引：46
作者：
MA, TH
FRIGERI, A
SKACH, W
VERKMAN, AS
机构：
[1] UNIV CALIF SAN FRANCISCO,DEPT PHYSIOL,SAN FRANCISCO,CA 94143
[2] UNIV CALIF SAN FRANCISCO,INST CARDIOVASC RES,SAN FRANCISCO,CA 94143
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 197卷 / 02期
关键词：
D O I：
10.1006/bbrc.1993.2529
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two kidney water channels have been identified: CHIP28 in proximal tubule and thin descending limb, and WCH-CD in collecting duct apical membrane. An homologous cDNA (WCH3) was obtained from rat kidney and found to encode a 276 amino acid, 29 kDa protein with 39% amino acid identity to rat CHIP28, 50% to WCH-CD and 49% to MIP26. The WCH3 transcript of 2.5 kb was expressed exclusively in kidney and was upregulated in dehydrated rats. Cell-free translation produced an ∼28 kDa protein. Analysis of the predicted amino acid sequence indicated a hydrophobic protein with 4-6 membrane-spanning domains, with one N-linked glycosylation site, two conserved NPA boxes common to MIP26 family proteins, and conserved residue C189 common to water channels. WCH3 is a new member of the MIP26 family of channel-forming proteins in mammalian kidney. © 1993 Academic Press. All rights reserved.
引用
收藏
页码：654 / 659
页数：6
相关论文
共 12 条
[1]   DEVELOPMENTAL GENE-EXPRESSION AND TISSUE DISTRIBUTION OF THE CHIP28 WATER-CHANNEL PROTEIN [J].
BONDY, C ;
CHIN, E ;
SMITH, BL ;
PRESTON, GM ;
AGRE, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (10) :4500-4504
[2]  
HASEGAWA H, 1993, IN PRESS AM J PHYSL
[3]  
HASEGAWA H, 1993, AM J PHYSIOL, V264, P2938
[4]  
MA T, 1993, IN PRESS J BIOL CHEM, V268
[5]   CHIP28 WATER CHANNELS ARE LOCALIZED IN CONSTITUTIVELY WATER-PERMEABLE SEGMENTS OF THE NEPHRON [J].
NIELSEN, S ;
SMITH, BL ;
CHRISTENSEN, EI ;
KNEPPER, MA ;
AGRE, P .
JOURNAL OF CELL BIOLOGY, 1993, 120 (02) :371-383
[6]   APPEARANCE OF WATER CHANNELS IN XENOPUS OOCYTES EXPRESSING RED-CELL CHIP28 PROTEIN [J].
PRESTON, GM ;
CARROLL, TP ;
GUGGINO, WB ;
AGRE, P .
SCIENCE, 1992, 256 (5055) :385-387
[7]   ISOLATION OF THE CDNA FOR ERYTHROCYTE INTEGRAL MEMBRANE-PROTEIN OF 28-KILODALTONS - MEMBER OF AN ANCIENT CHANNEL FAMILY [J].
PRESTON, GM ;
AGRE, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (24) :11110-11114
[8]   LOCALIZATION OF THE CHIP28 WATER CHANNEL IN RAT-KIDNEY [J].
SABOLIC, I ;
VALENTI, G ;
VERBAVATZ, JM ;
VANHOEK, AN ;
VERKMAN, AS ;
AUSIELLO, DA ;
BROWN, D .
AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 263 (06) :C1225-C1233
[9]  
VANHOEK AN, 1992, J BIOL CHEM, V267, P18267
[10]  
VERKMAN AS, 1993, WATER CHANNELS, P33
12