EFFECT OF GROWTH-FACTORS AND CYTOKINES ON EXPRESSION OF AMYLOID BETA-PROTEIN PRECURSOR MESSENGER-RNAS IN CULTURED NEURAL CELLS

被引：53

作者：

OHYAGI, Y ^{[1
]}

TABIRA, T ^{[1
]}

机构：

[1] NATL CTR NEUROL & PSYCHIAT, NATL INST NEUROSCI, DIV DEMYELINATING DIS & AGING, 4-1-1 OGAWA HIGASHI, KODAIRA, TOKYO 187, JAPAN

来源：

MOLECULAR BRAIN RESEARCH | 1993年 / 18卷 / 1-2期

关键词：

NEURON; ASTROCYTE; AMYLOID BETA-PROTEIN; GROWTH FACTOR; CYTOKINE; ALZHEIMERS DISEASE;

D O I：

10.1016/0169-328X(93)90181-N

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We analyzed the effect of several growth factors and cytokines on the expression of amyloid beta protein precursor (APP) mRNAs in cultured mouse neuronal and glial cells. In neuronal cultures from embryonic day-15 brain, Northern blotting revealed that APP mRNAs increased by 1.3- to 2.6-fold when treated with nerve growth factor, basic fibroblast growth factor, interleukin 1, interleukin 2, interleukin 3, interleukin 6 or granulocyte-macrophage colony-stimulating factor but not with tumor necrosis factor alpha. An S1 nuclease protection assay revealed that the enhanced APP mRNA in neuronal cultures was exclusively APP695 mRNA. On the other hand. astrocyte-enriched cultures prepared from postnatal day-2 brain did not show any significant alteration among these factors. We conclude that certain growth factors and cytokines could enhance APP 695 mRNA expression in neurons in vitro.

引用

页码：127 / 132

页数：6

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