THE EFFECT OF COLONY AGE ON PCR FINGERPRINTING

被引：25

作者：

COUTINHO, HLC ^{[1
]}

HANDLEY, BA ^{[1
]}

KAY, HE ^{[1
]}

STEVENSON, L ^{[1
]}

BERINGER, JE ^{[1
]}

机构：

[1] UNIV BRISTOL,SCH BIOL SCI,BRISTOL BS8 1UG,ENGLAND

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1993年 / 17卷 / 06期

关键词：

D O I：

10.1111/j.1472-765X.1993.tb01467.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The pattern of DNA fragments produced by the polymerase chain reaction using arbitrary primers is strongly influenced by the age of the colonies sampled. We show that the number, clarity and reproducibility of the bands produced is optimal when very young colonies are sampled.

引用

页码：282 / 284

页数：3

共 10 条

[1]

BERINGER JE, 1974, J GEN MICROBIOL, V84, P188

[2]

BERINGER JE, 1984, SOC APPLIED BACTERIO, V19, P79

[3] ARBITRARY PRIMER POLYMERASE CHAIN-REACTION, A POWERFUL METHOD TO IDENTIFY BACILLUS-THURINGIENSIS SEROVARS AND STRAINS [J].