EFFECTS OF COLCHICINE AND CYTOCHALASIN-B ON UPTAKE AND DEGRADATION OF ASIALO-GLYCOPROTEINS IN ISOLATED RAT HEPATOCYTES

被引：118

作者：

KOLSET, SO

TOLLESHAUG, H

BERG, T

机构：

[1] Institute for Nutrition Research, University of Oslo, Blindern

来源：

EXPERIMENTAL CELL RESEARCH | 1979年 / 122卷 / 01期

关键词：

D O I：

10.1016/0014-4827(79)90570-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have studied the effects of colchicine, an inhibitor of microtubular function, and cytochalasin B (CB), an inhibitor of microfilaments, on the uptake and degradation of asialo-glycoproteins in isolated rat hepatocytes. CB inhibited degradation only, while colchicine inhibited uptake as well as degradation. When the two were combined, no additive effect on degradation was found. The inhibition of uptake by colchicine could be accounted for by a reduction in the binding capacity of the plasma membrane for asialo-glycoprotein. The intracellular distribution of endocytosed asialo-glycoprotein was examined by isopycnic centrifugation in sucrose gradients. The results suggest that in cells treated with colchicine or CB, access of the endocytosed protein to the lysosomes is impeded. © 1979.

引用

页码：159 / 167

页数：9

共 38 条

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