ANTI-NEOPLASTIC AGENTS .3. EFFECTS OF DIBROMOETHYL AND VINYL ESTERS OF "N-BENZYLOXYCARBONYL-L-PHENYLALANINE ON EHRLICH ASCITES TUMOR-CELL METABOLISM

Evidence is presented that N‐benzyloxycarbonyl‐L‐phenylalanine vinyl ester and 1,2‐dibromoethyl ester are inhibitors of Walker 256 carcinosarcoma and Ehrlich ascites carcinoma tumor growth. The major effects of these two agents on Ehrlich ascites cell metabolism were the inhibition of deoxyribonucleic acid and protein synthesis and the alteration of cellular regulatory processes controlling cytokinetics. Deoxynucleotide (purine) kinase enzymes appeared to be the focal site for inhibition of deoxyribonucleic acid synthesis with marginal inhibition of thymidylate synthetase activity. Cyclic adenosine monophosphate levels were elevated by drug treatment whereas chromatin protein phosphorylation, cell respiration, and lysosomal activities were inhibited. N‐Benzyloxycarbonyl‐L‐phenylalanine 1,2‐dibromoethyl ester was a latent in vitro chymotrypsin inhibitor. Some preliminary evidence suggests that these activated esters may inhibit cellular enzymatic activity by alkylating imidazole and lysine residues of proteins. Copyright © 1978 Wiley‐Liss, Inc., A Wiley Company