BLOCK OF P-TYPE CA2+ CHANNELS IN FRESHLY DISSOCIATED RAT CEREBELLAR PURKINJE NEURONS BY DILTIAZEM AND VERAPAMIL

被引：30
|
作者：
ISHIBASHI, H
YATANI, A
AKAIKE, N
机构：
[1] KYUSHU UNIV,FAC MED,DEPT PHYSIOL,FUKUOKA 81282,JAPAN
[2] UNIV CINCINNATI,COLL MED,DEPT PHARMACOL & CELL BIOPHYS,CINCINNATI,OH 45267
来源：
BRAIN RESEARCH | 1995年 / 695卷 / 01期
关键词：
RAT CEREBELLAR PURKINJE NEURON; NYSTATIN-PERFORATED PATCH-CLAMP; P-TYPE CA2+ CHANNEL; DILTIAZEM; VERAPAMIL;
D O I：
10.1016/0006-8993(95)00815-8
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
We investigated the effects of organic Ca2+ channel blockers, diltiazem and verapamil, on the high voltage-activated P-type Ca2+ channels in freshly isolated rat Purkinje neurons. Both diltiazem and verapamil blocked P-type Ca2+ channel current without any change in the current-voltage relation. The block was concentration-dependent. In the presence of these agents, the inactivation curve was shifted to hyperpolarizing potentials. The characteristics of block of P-type Ca2+ channels by diltiazem and verapamil are similar to that of L-type Ca2+ channels. These results indicate that both benzothiazepine and phenylalkylamine react with P-type Ca2+ channels and suggest that some structural features common to which operate in both L-type and P-type Ca2+ channels may be involved in drug binding to these channels.
引用
收藏
页码：88 / 91
页数：4
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