MASS-PRODUCTION OF GINSENG (PANAX-GINSENG) EMBRYOIDS ON MEDIA CONTAINING HIGH-CONCENTRATIONS OF SUGAR

被引:13
作者
ASAKA, I [1 ]
II, I [1 ]
HIROTANI, M [1 ]
ASADA, Y [1 ]
YOSHIKAWA, T [1 ]
FURUYA, T [1 ]
机构
[1] KITASATO UNIV, SCH PHARMACEUT SCI, MINATO KU, TOKYO 108, JAPAN
关键词
PANAX GINSENG; ARALIACEAE; TISSUE CULTURE; EMBRYOID; PROPAGATION; SUGAR;
D O I
10.1055/s-2006-959438
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A lot of ginseng embryoids were produced by culturing on high concentrations of sugar media from the embryogenic tissues obtained by moderately high temperature treatment. When the sucrose concentration was 100 g/l, the number of embryoids produced were over 100 pieces per g of inoculum weight. It was about ten times of that produced by culturing on 30 g/l of sucrose. Glucose showed an effect similar to sucrose on the basis of weight percentage. However, mannitol did not show this effect. The embryoids obtained by these processes redifferentiated to normal plantlets on culturing on the medium containing 30 g/l of sucrose. The saponin components of the tissue containing embryoids showed a similar pattern to those of natural ginseng by HPLC.
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页码:146 / 148
页数:3
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