ALTERATION OF THE PROTEOGLYCAN FORM OF MACROPHAGE-COLONY-STIMULATING FACTOR PRODUCED BY A HUMAN STROMAL LINE STIMULATED BY TUMOR-NECROSIS-FACTOR-ALPHA

Immunoblot analysis of macrophage colony-stimulating factor (M-CSF) in KM 102 cell-conditioned medium showed the presence of two M-CSF molecular types, one being 85-kd M-CSF, the other a proteoglycan form (PG-M-CSF) carrying a chondroitin sulfate chain of variable length. When KM 102 cells were stimulated by TNF-cu, they produced more M-CSF than that produced in unstimulated condition, in which PG M-CSF had a shorter chondroitin sulfate chain. Although PGM-CSF has binding affinity for type V collagen, the PGM-CSF with the shorter chondroitin sulfate chain shows lower affinity. This spreads in type V collagen-containing agarose gel more easily than does PG-M-CSF with a longer chondroitin sulfate chain.