CHARACTERIZATION OF 2 SOLUBLE METALLOEXOPEPTIDASES IN THE PROTOZOAN PARASITE LEISHMANIA-MAJOR

被引：9

作者：

SCHNEIDER, P ^{[1
]}

GLASER, TA ^{[1
]}

机构：

[1] UNIV LAUSANNE,INST BIOCHEM,CH-1066 EPALINGES,SWITZERLAND

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1993年 / 62卷 / 02期

关键词：

LEISHMANIA; AMINOPEPTIDASE; CARBOXYPEPTIDASE; PROTEASE; BESTATIN; PROMASTIGOTE; AMASTIGOTE;

D O I：

10.1016/0166-6851(93)90111-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two soluble exopeptidases were identified in promastigotes of Leishmania major, using an iodinated model tetrapeptide (LIAY) as substrate. Similar activities were also detected in L. major amastigotes and in different species of Leishmania promastigotes. A carboxy- and an aminopeptidase activity were resolved and isolated by anion exchange and gel permeation chromatographies. A single polypeptide of 62 kDa co-purified with the aminopeptidase activity. Optimum pH was neutral for the carboxypeptidase and neutral to alkaline for the aminopeptidase. Both activities were able to hydrolyse a dipeptide substrate (YL), and were inhibited by 20 mu M bestatin and 200 mu M I,10-phenanthroline, but not by leupeptin, iodoacetamide and a range of other inhibitors. These results strongly suggest that both enzymes are metalloexopeptidases and thus represent a novel class of soluble peptidases in Leishmania.

引用

页码：223 / 231

页数：9

共 28 条

[11]

BOUVIER J, 1985, J BIOL CHEM, V260, P5504

[12] IDENTIFICATION OF THE PROMASTIGOTE SURFACE PROTEASE IN 7 SPECIES OF LEISHMANIA [J].

BOUVIER, J ;

ETGES, R ;

BORDIER, C .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1987, 24 (01) :73-79

[13] LEUCINE AMINOPEPTIDASE - BESTATIN INHIBITION AND A MODEL FOR ENZYME-CATALYZED PEPTIDE HYDROLYSIS [J].

BURLEY, SK ;

DAVID, PR ;

LIPSCOMB, WN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (16) :6916-6920

[14] IDENTIFICATION OF A SURFACE METALLOPROTEINASE ON 13 SPECIES OF LEISHMANIA ISOLATED FROM HUMANS, CRITHIDIA-FASCICULATA, AND HERPETOMONAS-SAMUELPESSOAI [J].

ETGES, R .

ACTA TROPICA, 1992, 50 (03) :205-217

[15] INTRACELLULAR DIFFERENTIATION OF LEISHMANIA-AMAZONENSIS PROMASTIGOTES TO AMASTIGOTES - PRESENCE OF MEGASOMES, CYSTEINE PROTEINASE ACTIVITY AND SUSCEPTIBILITY TO LEUCINEMETHYL ESTER [J].

GALVAOQUINTAO, L ;

ALFIERI, SC ;

RYTER, A ;

RABINOVITCH, M .

PARASITOLOGY, 1990, 101 :7-13

[16] LEISHMANIA-MAJOR AND LEISHMANIA-DONOVANI - A METHOD FOR RAPID PURIFICATION OF AMASTIGOTES [J].

GLASER, TA ;

WELLS, SJ ;

SPITHILL, TW ;

PETTITT, JM ;

HUMPHRIS, DC ;

MUKKADA, AJ .

EXPERIMENTAL PARASITOLOGY, 1990, 71 (03) :343-345

[17] LEISHMANIAL PROTEIN-KINASES PHOSPHORYLATE COMPONENTS OF THE COMPLEMENT-SYSTEM [J].

HERMOSO, T ;

FISHELSON, Z ;

BECKER, SI ;

HIRSCHBERG, K ;

JAFFE, CL .

EMBO JOURNAL, 1991, 10 (13) :4061-4067

[18] HYDROLYSIS OF L-LEUCINE METHYL-ESTER BY LEISHMANIA-MEXICANA-MEXICANA AMASTIGOTE CYSTEINE PROTEINASES [J].

HUNTER, CA ;

LOCKWOOD, BC ;

COOMBS, GH .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1992, 22 (06) :711-717

[19] CRITHIDIA-FASCICULATA CONTAINS A TRANSCRIBED LEISHMANIAL SURFACE PROTEINASE (GP63) GENE HOMOLOG [J].

INVERSO, JA ;

MEDINAACOSTA, E ;

OCONNOR, J ;

RUSSELL, DG ;

CROSS, GAM .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1993, 57 (01) :47-54

[20] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

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