SEPARATION OF THE ALPHA-TOCOPHEROL MODEL-COMPOUND 2,2,5,7,8-PENTAMETHYL-6-CHROMANOL FROM ITS OXIDATION-PRODUCTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：7
作者：
BACA, M
SUARNA, C
SOUTHWELLKEELY, PT
机构：
[1] University of New South Wales, Kensington, New South Wales 2033
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY | 1991年 / 14卷 / 10期
关键词：
D O I：
10.1080/01483919108049665
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid method is described for the separation of the alpha-tocopherol model compound, 2,2,5,7,8-pentamethyl-6-chromanol (6), from 9 of its oxidation products in a single 35 minute run. Separated derivatives of 6, in order of elution, included the 5-cholesteroxymethyl (1), spirotrimer (2), spirodimer (3), 5-formyl (4), 5-ethoxymethyl (5), dihydroxydimer (7), chroman dione (8), quinone (9) and pyrano xanthene (10). A normal phase system, using gradient elution is employed, the eluent being monitored at 290 nm. The minimum detection limit for compounds 1-8 was 0.1-mu-g per injection and for compounds 9 and 10 it was 0.3-mu-g per injection.
引用
收藏
页码：1957 / 1966
页数：10
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