CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF THE ENGRAILED HOMEODOMAIN AND OF AN ENGRAILED HOMEODOMAIN DNA COMPLEX

被引：15

作者：

LIU, BS ^{[1
]}

KISSINGER, CR ^{[1
]}

PABO, CO ^{[1
]}

MARTINBLANCO, E ^{[1
]}

KORNBERG, TB ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,BIOCHEM & BIOPHYS,SAN FRANCISCO,CA 94143

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 171卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91385-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The homeodomain from the engrailed protein of Drosophila has been crystallized from ammonium phosphate at pH 6.8. The crystals form in space group P6122 (or P6522), with cell dimensions a = b = 44.8 Å and c = 118.2 Å. These crystals diffract to 1.8 Å resolution. A complex containing the engrailed homeodomain and a duplex DNA site also has been crystallized. The cocrystals form in space group C2 with a = 131.2 Å, b = 45.5 Å, c = 72.9 Å and β = 119.0°. These crystals diffract to 2.6 Å resolution. © 1990.

引用

页码：257 / 259

页数：3

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