INHIBITION OF THE NADP-LINKED GLUTAMATE-DEHYDROGENASE FROM TRYPANOSOMA-CRUZI BY SULFHYDRYL-REAGENTS

被引：9

作者：

JUAN, SM

CAZZULO, JJ

SEGURA, EL

机构：

[1] NATL UNIV ROSARIO,FDN MIGUEL LILLO,CONICET,CTR ESTUDIOS FOTOSINTET & BIOQUIM,ROSARIO 2000,ARGENTINA

[2] ESTADO SALUD PUBL,INST NACL DIAG & INVEST ENFERMEDAD CHAGAS DR MARIO FATALA CHABEN,BUENOS AIRES 1063,ARGENTINA

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1979年 / 63卷 / 04期

关键词：

D O I：

10.1016/0305-0491(79)90059-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. The NADP-linked glutamate dehydrogenase purified from epimastigotes of Trypanosoma cruzi was strongly, but not completely, inhibited by sulfhydryl reagents, in the presence of Tris-HCl or phosphate buffers. 2. 2. The enzyme modified by preincubation with o-iodosobenzoate had a kinetic behaviour different from that shown by the enzyme modified with other inhibitors, such as N-ethylmaleimide or p-chloromercuribenzoate. 3. 3. The inhibition by o-iodosobenzoate was additive with the inhibition by the other reagents tested. 4. 4. It is suggested that two or more different sulfhydryl groups, placed probably near the active site, are involved in these effects. © 1979.

引用

页码：531 / 535

页数：5

共 8 条

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