IDENTIFICATION OF PLASMINOGEN-ACTIVATOR IN OSTEOCLASTS

被引：27
作者：
GRILLS, BL
GALLAGHER, JA
ALLAN, EH
YUMITA, S
MARTIN, TJ
机构：
[1] ST VINCENTS INST MED RES, 41 VICTORIA PARADE, MELBOURNE 3065, AUSTRALIA
[2] UNIV LIVERPOOL, DEPT HUMAN ANAT & CELL BIOL, LIVERPOOL L69 3BX, ENGLAND
来源：
JOURNAL OF BONE AND MINERAL RESEARCH | 1990年 / 5卷 / 05期
关键词：
D O I：
10.1002/jbmr.5650050512
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Plasminogen activator (PA) was located in newborn rat osteoclasts using a single‐cell assay. Immunohistochemistry using biotin‐streptavidin‐peroxidase indicated the presence of both tissue‐type plasminogen activator (tPA) and urokinase (uPA) within the cytoplasm of osteoclasts isolated from newborn rat long bones. Electron microscopic immunohistochemistry using the biotin‐streptavidin‐colloidal gold system on L.R. Gold thin resin sections of undecalcified, newborn rat tibial metaphyseal trabecular bone identified these proteases in the lysosomal network of osteoclasts. uPA was also localized in marrow macrophage lysosomes, but tPA was not detected in these cells. The localization of these enzymes within osteoclasts may imply their involvement in bone resorption. Copyright © 1990 ASBMR
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页码：499 / 505
页数：7
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