PENICILLIN-G PRODUCTION BY IMMOBILIZED WHOLE CELLS OF PENICILLIUM-CHRYSOGENUM

被引：64

作者：

MORIKAWA, Y ^{[1
]}

KARUBE, I ^{[1
]}

SUZUKI, S ^{[1
]}

机构：

[1] TOKYO INST TECHNOL, RESOURCES UTILIZAT RES LAB, NAGATSUTA CHO, MIDORI KU, YOKOHAMA 227, JAPAN

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1979年 / 21卷 / 02期

关键词：

D O I：

10.1002/bit.260210211

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Penicillium chrysogenum was immobilized in polyacrylamide gel prepared from 5% acrylamide monomers (85% acrylamide and 15% N,N′‐methylene bisacrylamide). Penicillin produced from glucose by the immobilized mycelium was 17% of that produced by washed mycelium. However, the activity of penicillin production of the washed mycelium decreased with repeated use. On the other hand, the activity of the immobilized mycelium increased initially and decreased gradually with repeated use. The rate of oxygen uptake of the immobilized mycelium was about 30% of that of the washed mycelium. The immobilized mycelium required oxygen for the production of penicillin. Copyright © 1979 John Wiley & Sons, Inc.

引用

页码：261 / 270

页数：10

共 17 条

[1] IMMOBILIZED ASPARTASE-CONTAINING MICROBIAL CELLS - PREPARATION AND ENZYMATIC PROPERTIES [J].

CHIBATA, I ;

TOSA, T ;

SATO, T .

APPLIED MICROBIOLOGY, 1974, 27 (05) :878-885

[2] INHIBITION OF PENICILLIN FORMATION BY AMINO ACID ANALOGS [J].

DEMAIN, AL .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1956, 64 (01) :74-79

[3]

GORDON AH, 1969, ELECTROPHORESIS PROT

[4] BIOSYNTHESIS OF PENICILLIN .4. SYNTHESIS OF BENZYLPENICILLIN BY WASHED MYCELIUM OF PENICILLIUM-CHRYSOGENUM [J].

HALLIDAY, WJ ;

ARNSTEIN, HRV .

BIOCHEMICAL JOURNAL, 1956, 64 (02) :380-384

[5] THE ROLE OF THE CONSTITUENTS OF SYNTHETIC MEDIA FOR PENICILLIN PRODUCTION [J].

JARVIS, FG ;

JOHNSON, MJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1947, 69 (12) :3010-3017

[6]

KARUBE I, 1977, J FERMENT TECHNOL, V55, P243

[7] BIOCHEMICAL FUEL-CELL UTILIZING IMMOBILIZED CELLS OF CLOSTRIDIUM-BUTYRICUM [J].

KARUBE, I ;

MATSUNAGA, T ;

TSURU, S ;

SUZUKI, S .

BIOTECHNOLOGY AND BIOENGINEERING, 1977, 19 (11) :1727-1733

[8] CONTINUOUS HYDROGEN PRODUCTION BY IMMOBILIZED WHOLE CELLS OF CLOSTRIDIUM-BUTYRICUM [J].

KARUBE, I ;

MATSUNAGA, T ;

TSURU, S ;

SUZUKI, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 444 (02) :338-343

[9] MICROBIAL ELECTRODE BOD SENSORS [J].

KARUBE, I ;

MATSUNAGA, T ;

MITSUDA, S ;

SUZUKI, S .

BIOTECHNOLOGY AND BIOENGINEERING, 1977, 19 (10) :1535-1547

[10]

Karube I., 1977, J SOL-PHASE BIOCHEM, V2, P97

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