ISOLATION AND SEQUENCE DETERMINATION OF GALANIN FROM THE PITUITARY OF YELLOWFIN TUNA

被引：26

作者：

HABU, A ^{[1
]}

OHISHI, T ^{[1
]}

MIHARA, S ^{[1
]}

OHKUBO, R ^{[1
]}

HONG, YM ^{[1
]}

MOCHIZUKI, T ^{[1
]}

YANAIHARA, N ^{[1
]}

机构：

[1] APRO SCI INC,INST LIFE SCI,TOKUSHIMA 722,JAPAN

来源：

BIOMEDICAL RESEARCH-TOKYO | 1994年 / 15卷 / 06期

关键词：

D O I：

10.2220/biomedres.15.357

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Galanin-like immunoreactivity (91.3 pmol/g frozen tissue) was detected by a radioimmunoassay using anti-galanin(1-15) serum in the pituitary extract of yellowfin tuna. The peptide with immunoreactive galanin was isolated by several purification steps, using ion exchange chromatography and reverse phase HPLC. Tuna galanin comprised 29 amino acid residues and contained an a-amidated C-terminal residues. Its complete amino acid sequence is: H-Gly-Trp-Thr-Leu-Asn-Ala-Ala-Gly-Tyr-Leu-Leu-Gly-Pro-His-Gly-Ile-Asp-Gly- His-Arg-Thr-Leu-Gly-Asp-Lys-Pro-Gly-Leu-Ala-NH2. Two substitutions were found in the N-terminal 1-15 sequence of tuna galanin, although the 1-15 sequence was conserved in mammalian and avian galanins.

引用

页码：357 / 362

页数：6

共 15 条

[1] NEUROPEPTIDES IN THE AMPHIBIAN BRAIN [J].