Complete Genome Sequence of the Engineered Escherichia coli SHuffle Strains and Their Wild-Type Parents

被引：12

作者：

Anton, Brian P. ^{[1
]}

Fomenkov, Alexey ^{[1
]}

Raleigh, Elisabeth A. ^{[1
]}

Berkmen, Mehmet ^{[1
]}

机构：

[1] New England Biolabs Inc, Ipswich, MA 01938 USA

来源：

GENOME ANNOUNCEMENTS | 2016年 / 4卷 / 02期

关键词：

D O I：

10.1128/genomeA.00230-16

中图分类号：

学科分类号：

摘要：

SHuffle strains are genetically engineered Escherichia coli strains that are capable of oxidizing cysteines within proteins to form disulfide bonds. Here we present the complete genome of both the K-12 and B versions of SHuffle strains along with their parental ancestors. These strains have been of significant use to both the general scientific community and the biotech industry, interested in producing novel disulfide-bonded proteins that were hitherto unable to be expressed in standard E. coli expression strains.

引用

页数：2

共 6 条

[1] Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm
Bessette, PH
Åslund, F
Beckwith, J
Georgiou, G
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (24) : 13703 - 13708
[2] Towards single-copy gene expression systems making gene cloning physiologically relevant:: Lambda InCh, a simple Escherichia coli plasmid-chromosome shuttle system
Boyd, D
Weiss, DS
Chen, JC
Beckwith, J
[J]. JOURNAL OF BACTERIOLOGY, 2000, 182 (03) : 842 - 847
[3] Ke Na, 2014, Curr Protoc Mol Biol, V108, DOI 10.1002/0471142727.mb1601bs108
[4] SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm
Lobstein, Julie
Emrich, Charlie A.
Jeans, Chris
Faulkner, Melinda
Riggs, Paul
Berkmen, Mehmet
[J]. MICROBIAL CELL FACTORIES, 2012, 11
[5] Conversion of a peroxiredoxin into a disulfide reductase by a triplet repeat expansion
Ritz, D
Lim, J
Reynolds, CM
Poole, LB
Beckwith, J
[J]. SCIENCE, 2001, 294 (5540) : 158 - 160
[6] Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria
Robinson, Michael-Paul
Ke, Na
Lobstein, Julie
Peterson, Cristen
Szkodny, Alana
Mansell, Thomas J.
Tuckey, Corinna
Riggs, Paul D.
Colussi, Paul A.
Noren, Christopher J.
Taron, Christopher H.
DeLisa, Matthew P.
Berkmen, Mehmet
[J]. NATURE COMMUNICATIONS, 2015, 6

← 1 →