A SIMPLIFIED RAPID METHOD FOR RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM ANALYSIS AFTER BONE-MARROW TRANSPLANTATION

被引：9

作者：

SCHREINER, T

WIESNETH, M

MACCARI, B

SAWODNY, B

PROCHNOWCALZIA, H

KUBANEK, B

机构：

[1] UNIV ULM,RED CROSS,CTR BLOOD,ULM,GERMANY

[2] UNIV ULM,DEPT TRANSFUS MED,ULM,GERMANY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 168卷 / 02期

关键词：

RESTRICTION FRAGMENT LENGTH POLYMORPHISM; CHIMERISM ANALYSIS; BONE MARROW TRANSPLANTATION; (METHOD);

D O I：

10.1016/0022-1759(94)90053-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and simple protocol for restriction fragment length polymorphism (RFLP) analysis using digoxigenin labeled DNA probes is presented. The method described gives consistently good results even with minute cell numbers. The method has been used to evaluate chimerism after bone marrow transplantation but could also find other applications.

引用

页码：183 / 185

页数：3

共 6 条

[1] HLA-DR ALLOGENOTYPING USING EXON-SPECIFIC CDNA PROBES AND APPLICATION OF RAPID MINIGEL METHODS [J].