OVERLAPPING DISTRIBUTION OF 2 GLYCOSYLTRANSFERASES IN THE GOLGI-APPARATUS OF HELA-CELLS

被引：196

作者：

NILSSON, T ^{[1
]}

PYPAERT, M ^{[1
]}

HOE, MH ^{[1
]}

SLUSAREWICZ, P ^{[1
]}

BERGER, EG ^{[1
]}

WARREN, G ^{[1
]}

机构：

[1] UNIV ZURICH,INST PHYSIOL,CH-8057 ZURICH,SWITZERLAND

来源：

JOURNAL OF CELL BIOLOGY | 1993年 / 120卷 / 01期

关键词：

D O I：

10.1083/jcb.120.1.5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Thin, frozen sections of a HeLa cell line were double labeled with specific antibodies to localize the trans-Golgi enzyme, beta1,4 galactosyltransferase (GalT) and the medial enzyme, N-acetylglucosaminylransferase I (NAGT I). The latter was detected by generating a HeLa cell line stably expressing a myc-tagged version of the endogenous protein. GalT was found in the trans-cisterna and trans-Golgi network but, contrary to expectation, NAGT I was found both in the medial- and trans-cisternae, overlapping the distribution of GalT. About one third of the NAGT I and half of the GalT were found in the shared, trans-cisterna. These data show that the differences between cisternae are determined not by different sets of enzymes but by different mixtures.

引用

页码：5 / 13

页数：9

共 47 条

[1] GOLGI RETENTION OF A TRANS-GOLGI MEMBRANE-PROTEIN, GALACTOSYL-TRANSFERASE, REQUIRES CYSTEINE AND HISTIDINE-RESIDUES WITHIN THE MEMBRANE-ANCHORING DOMAIN [J].