A MODIFIED METHOD TO DIRECTLY DETECT IN SDS-PAGE THE BACTERIOCIN OF PEDIOCOCCUS-ACIDILACTICI

被引：42

作者：

BHUNIA, AK ^{[1
]}

JOHNSON, MG ^{[1
]}

机构：

[1] UNIV ARKANSAS,ARKANSAS BIOTECHNOL CTR,FAYETTEVILLE,AR 72703

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1992年 / 15卷 / 01期

关键词：

D O I：

10.1111/j.1472-765X.1992.tb00709.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A modified direct detection system was developed to identify the antimicrobial peptide, pediocin AcH in SDS-PAGE. The gel containing pediocin AcH was stained for 30 min, destained for 1.5 h, and then overlaid with indicator bacteria. The pediocin AcH band was identified by a zone of inhibition of bacterial growth surrounding the stained protein band.

引用

页码：5 / 7

页数：3

共 14 条

[1] PURIFICATION AND CHARACTERIZATION OF THE LACTOBACILLUS-ACIDOPHILUS BACTERIOCIN LACTACIN-B [J].

BAREFOOT, SF ;

KLAENHAMMER, TR .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1984, 26 (03) :328-334

[2] PURIFICATION, CHARACTERIZATION AND ANTIMICROBIAL SPECTRUM OF A BACTERIOCIN PRODUCED BY PEDIOCOCCUS-ACIDILACTICI [J].

BHUNIA, AK ;

JOHNSON, MC ;

RAY, B .

JOURNAL OF APPLIED BACTERIOLOGY, 1988, 65 (04) :261-268

[3] MODE OF ACTION OF PEDIOCIN ACH FROM PEDIOCOCCUS-ACIDILACTICI-H ON SENSITIVE BACTERIAL STRAINS [J].

BHUNIA, AK ;

JOHNSON, MC ;

RAY, B ;

KALCHAYANAND, N .

JOURNAL OF APPLIED BACTERIOLOGY, 1991, 70 (01) :25-33

[4] DIRECT DETECTION OF AN ANTIMICROBIAL PEPTIDE OF PEDIOCOCCUS-ACIDILACTICI IN SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS [J].

BHUNIA, AK ;

JOHNSON, MC ;

RAY, B .

JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1987, 2 (05) :319-322

[5] ASSOCIATION OF A 13.6-MEGADALTON PLASMID IN PEDIOCOCCUS-PENTOSACEUS WITH BACTERIOCIN ACTIVITY [J].

DAESCHEL, MA ;

KLAENHAMMER, TR .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1985, 50 (06) :1538-1541

[6] MICROBIAL INHIBITION BY AN ISOLATE OF PEDIOCOCCUS FROM CUCUMBER BRINES [J].

FLEMING, HP ;

ETCHELLS, JL ;

COSTILOW, RN .

APPLIED MICROBIOLOGY, 1975, 30 (06) :1040-1042

[7] PLASMID-ASSOCIATED BACTERIOCIN PRODUCTION AND SUCROSE FERMENTATION IN PEDIOCOCCUS-ACIDILACTICI [J].

GONZALEZ, CF ;

KUNKA, BS .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (10) :2534-2538

[8] A BACTERIOCIN PRODUCED BY PEDIOCOCCUS SPECIES ASSOCIATED WITH A 5.5-MEGADALTON PLASMID [J].

HOOVER, DG ;

WALSH, PM ;

KOLAETIS, KM ;

DALY, MM .

JOURNAL OF FOOD PROTECTION, 1988, 51 (01) :29-31

[9] ANTAGONISTIC EFFECTS OF LACTOBACILLI AND PEDIOCOCCI TO CONTROL INTESTINAL COLONIZATION BY HUMAN ENTEROPATHOGENS IN LIVE POULTRY [J].

JUVEN, BJ ;

MEINERSMANN, RJ ;

STERN, NJ .

JOURNAL OF APPLIED BACTERIOLOGY, 1991, 70 (02) :95-103

[10] VIABILITY LOSS OF FOODBORNE PATHOGENS BY STARTER CULTURE METABOLITES [J].

MOTLAGH, AM ;

JOHNSON, MC ;

RAY, B .

JOURNAL OF FOOD PROTECTION, 1991, 54 (11) :873-&

← 1 2 →