EFFECTS OF LITHIUM ION ON ADP RIBOSYLATION OF INHIBITORY GTP-BINDING PROTEIN BY PERTUSSIS TOXIN, ISLET-ACTIVATING PROTEIN

被引：9
作者：
KAWAMOTO, H
WATANABE, Y
IMAIZUMI, T
IWASAKI, T
YOSHIDA, H
机构：
[1] OSAKA UNIV,SCH MED,DEPT PHARMACOL 1,4-3-57 NAKANOSHIMA,KITA KU,OSAKA 530,JAPAN
[2] HYOGO MED UNIV,DEPT MED 1,NISHINOMIYA,HYOGO 663,JAPAN
来源：
EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION | 1991年 / 206卷 / 01期
关键词：
LI+; GTP-BINDING PROTEIN (INHIBITORY); ADP RIBOSYLATION; ISLET-ACTIVATING PROTEIN; CARDIAC VENTRICULAR CELL (RAT);
D O I：
10.1016/0922-4106(91)90143-6
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Studies were made on the effects of Li+ on ADP ribosylation of inhibitory GTP-binding (G(i)) protein by islet-activating protein (IAP), pertussis toxin. The ADP ribosylation of 40-41 kDa proteins of the membranes of rat cardiac ventricular cells by IAP was reduced by the addition of a nonhydrolyzable analog of guanine nucleotide. GTP-gamma-S, indicating that these proteins included G(i) protein. The addition of LiCl (0.5-10 mM) to the membrane fractions of the cells attenuated the ADP ribosylation of the G(i) protein of the cell membranes by IAP dose-dependently. The effects of LiCl were reversible. Of the monovalent ions tested, Li+ inhibited the ADP ribosylation of the protein by IAP most strongly. The effects of LiCl (2 mM) were observed even in the presence of 150 mM KCl. Moreover, LiCl decreased the ADP ribosylation of purified G(i) protein by IAP. These results support that G(i) proteins are one of the targets for the therapeutic effects of lithium.
引用
收藏
页码：33 / 37
页数：5
相关论文
共 30 条
[1]   INCREASED BRAIN MYOINOSITOL 1-PHOSPHATE IN LITHIUM-TREATED RATS [J].
ALLISON, JH ;
BLISNER, ME ;
HOLLAND, WH ;
HIPPS, PP ;
SHERMAN, WR .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1976, 71 (02) :664-670
[2]   LITHIUM INHIBITS ADRENERGIC AND CHOLINERGIC INCREASES IN GTP BINDING IN RAT CORTEX [J].
AVISSAR, S ;
SCHREIBER, G ;
DANON, A ;
BELMAKER, RH .
NATURE, 1988, 331 (6155) :440-442
[3]  
BELMAKER RH, 1981, BIOL PSYCHIAT, V16, P333
[4]   LITHIUM AMPLIFIES AGONIST-DEPENDENT PHOSPHATIDYLINOSITOL RESPONSES IN BRAIN AND SALIVARY-GLANDS [J].
BERRIDGE, MJ ;
DOWNES, CP ;
HANLEY, MR .
BIOCHEMICAL JOURNAL, 1982, 206 (03) :587-595
[5]  
BOKOCH GM, 1984, J BIOL CHEM, V259, P3560
[6]  
CADE JFJ, 1949, MED J AUSTRALIA, V36, P349
[7]   CHRONIC LITHIUM TREATMENT REDUCES NOREPINEPHRINE-STIMULATED INOSITOL PHOSPHOLIPID HYDROLYSIS IN RAT CORTEX [J].
CASEBOLT, TL ;
JOPE, RS .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1987, 140 (02) :245-246
[8]  
CODINA J, 1984, J BIOL CHEM, V259, P5871
[9]   EFFECT OF NACL AND LICL ON VASOPRESSIN-SENSITIVE ADENYL CYCLASE [J].
DOUSA, T ;
HECHTER, O .
LIFE SCIENCE PART 1 PHYSIOLOGY & PHARMACOLOGY, 1970, 9 (13) :765-+
[10]   LITHIUM INHIBITION OF ADRENALINE-STIMULATED ADENYLATE CYCLASE IN HUMANS [J].
EBSTEIN, R ;
BELMAKER, R ;
GRUNHAUS, L ;
RIMON, R .
NATURE, 1976, 259 (5542) :411-413
123