ELECTROSPRAY IONIZATION COMBINED WITH ION TRAP MASS-SPECTROMETRY

Ions from a variety of molecules, formed via electrospray, have been Injected Into and analyzed with a quadrupole ion trap mass spectrometer. Examples are shown in which one or more stages of mass spectrometry (e.g., mass spectrometry/mass spectrometry) have been performed on both multiply charged anions and cations. Compounds for which data are described Include the disodium salt of 2-hydroxynaphthalene-3,6-disulfonic acid, Direct Red 81, bradykinin, melittin, cytochrome c, myoglobin, and bovine albumin. For some compounds, notably the sulfonates, evidence is presented for the injection of highly solvated Ions that desolvate within the ion trap. The cations derived from the peptides, on the other hand, appear to be essentially desolvated prior to injection Into the ion trap. © 1990, American Chemical Society. All rights reserved.