ANALYSIS OF AN ANAEROBICALLY INDUCED PROMOTER FOR THE COBALAMIN BIOSYNTHETIC GENES IN SALMONELLA-TYPHIMURIUM

被引：11

作者：

RICHTERDAHLFORS, AA ^{[1
]}

ANDERSSON, DI ^{[1
]}

机构：

[1] UNIV UPPSALA, CTR BIOMED, DEPT MICROBIOL, BOX 581, S-75123 UPPSALA, SWEDEN

来源：

MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 06期

关键词：

D O I：

10.1111/j.1365-2958.1991.tb00780.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified an anaerobically induced promoter for the cobalamin biosynthetic (cob) genes. In a plasmid the Cob promoter showed two of the three types of control of the intact chromosomal Cob operon: anaerobic induction and cAMP stimulation. Cobalamin repression was observed only in promoter fragments which included sequences far downstream of the transcription start site, suggesting that this control is post-transcriptional. One anaerobically induced transcript was identified and its 5' end was determined. Deletion mapping showed that 60 nucleotides upstream of the start site were sufficient for anaerobic synthesis of this transcript. Upstream of the transcription start site a putative sigma-70-dependent -10 recognition sequence was identified; however, no consensus -35 region was observed.

引用

页码：1337 / 1345

页数：9

共 47 条

[21] COBALAMIN (VITAMIN-B12) BIOSYNTHETIC GENES OF SALMONELLA-TYPHIMURIUM
JETER, RM
ROTH, JR
[J]. JOURNAL OF BACTERIOLOGY, 1987, 169 (07) : 3189 - 3198
[22] A LOW-COPY-NUMBER VECTOR UTILIZING BETA-GALACTOSIDASE FOR THE ANALYSIS OF GENE-CONTROL ELEMENTS
KOOP, AH
HARTLEY, ME
BOURGEOIS, S
[J]. GENE, 1987, 52 (2-3) : 245 - 256
[23] IDENTIFICATION AND LOCATION OF L-GLYCERATE, AN UNUSUAL ACYL SUBSTITUENT IN GELLAN GUM
KUO, MS
MORT, AJ
DELL, A
[J]. CARBOHYDRATE RESEARCH, 1986, 156 : 173 - 187
[24] MUTANTS OF ESCHERICHIA-COLI-K12 UNABLE TO USE FUMARATE AS AN ANAEROBIC ELECTRON-ACCEPTOR
LAMBDEN, PR
GUEST, JR
[J]. JOURNAL OF GENERAL MICROBIOLOGY, 1976, 97 (DEC): : 145 - 160
[25] LEONG JM, 1985, J BIOL CHEM, V260, P4468
[26] Maniatis T., 1982, MOL CLONING
[27] MUTATIONS AFFECTING REGULATION OF METHIONINE BIOSYNTHETIC GENES ISOLATED BY USE OF MET-LAC FUSIONS
MULLIGAN, JT
MARGOLIN, W
KRUEGER, JH
WALKER, GC
[J]. JOURNAL OF BACTERIOLOGY, 1982, 151 (02) : 609 - 619
[28] POSITIVE CONTROL OF TRANSCRIPTION INITIATION IN BACTERIA
RAIBAUD, O
SCHWARTZ, M
[J]. ANNUAL REVIEW OF GENETICS, 1984, 18 : 173 - 206
[29] FUNCTIONS REQUIRED FOR VITAMIN-B12-DEPENDENT ETHANOLAMINE UTILIZATION IN SALMONELLA-TYPHIMURIUM
ROOF, DM
ROTH, JR
[J]. JOURNAL OF BACTERIOLOGY, 1989, 171 (06) : 3316 - 3323
[30] ETHANOLAMINE UTILIZATION IN SALMONELLA-TYPHIMURIUM
ROOF, DM
ROTH, JR
[J]. JOURNAL OF BACTERIOLOGY, 1988, 170 (09) : 3855 - 3863

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