PROTEIN ENGINEERING AND THE DISSECTION OF PROTEIN-FOLDING PATHWAYS

被引：14
作者：
SERRANO, L
机构：
[1] European Molecular Biology Laboratory, Structures and Biocomputing Unit, Heidelberg, D6900
来源：
CURRENT OPINION IN STRUCTURAL BIOLOGY | 1994年 / 4卷 / 01期
关键词：
D O I：
10.1016/S0959-440X(94)90067-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The use of protein engineering in combination with fast kinetics to obtain information about the transition state and intermediates in the folding reaction of globular proteins is re-examined. This method yields information complementary to the quenched-flow NMR technique, but suffers from many of the same limitations. Combined use of quenched-flow NMR and protein engineering offers a picture of the kinetic folding intermediate that differs in some way from that of an equilibrium molten globule.
引用
收藏
页码：107 / 111
页数：5
相关论文
共 21 条
[1]   PULSED H/D-EXCHANGE STUDIES OF FOLDING INTERMEDIATES [J].
BALDWIN, RL .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 1993, 3 (01) :84-91
[2]   CHARACTERIZATION OF AN INTERMEDIATE IN THE FOLDING PATHWAY OF PHOSPHOGLYCERATE KINASE - CHEMICAL-REACTIVITY OF GENETICALLY INTRODUCED CYSTEINYL RESIDUES DURING THE FOLDING PROCESS [J].
BALLERY, N ;
DESMADRIL, M ;
MINARD, P ;
YON, JM .
BIOCHEMISTRY, 1993, 32 (02) :708-714
[3]   THE USE OF DOUBLE MUTANTS TO DETECT STRUCTURAL-CHANGES IN THE ACTIVE-SITE OF THE TYROSYL-TRANSFER RNA-SYNTHETASE (BACILLUS-STEAROTHERMOPHILUS) [J].
CARTER, PJ ;
WINTER, G ;
WILKINSON, AJ ;
FERSHT, AR .
CELL, 1984, 38 (03) :835-840
[4]   ENGINEERED DISULFIDE BONDS AS PROBES OF THE FOLDING PATHWAY OF BARNASE - INCREASING THE STABILITY OF PROTEINS AGAINST THE RATE OF DENATURATION [J].
CLARKE, J ;
FERSHT, AR .
BIOCHEMISTRY, 1993, 32 (16) :4322-4329
[5]   PROTEIN FOLDING PATHWAYS DETERMINED USING DISULFIDE BONDS [J].
CREIGHTON, TE .
BIOESSAYS, 1992, 14 (03) :195-200
[6]   DISSECTING THE DISULFIDE-COUPLED FOLDING PATHWAY OF BOVINE PANCREATIC TRYPSIN-INHIBITOR - FORMING THE 1ST DISULFIDE BONDS IN ANALOGS OF THE REDUCED PROTEIN [J].
DARBY, NJ ;
CREIGHTON, TE .
JOURNAL OF MOLECULAR BIOLOGY, 1993, 232 (03) :873-896
[7]   PROTEIN FOLDING STUDIED USING HYDROGEN-EXCHANGE LABELING AND 2-DIMENSIONAL NMR [J].
ENGLANDER, SW ;
MAYNE, L .
ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 1992, 21 :243-265
[8]   THE MOLTEN GLOBULE PROTEIN CONFORMATION PROBED BY DISULFIDE BONDS [J].
EWBANK, JJ ;
CREIGHTON, TE .
NATURE, 1991, 350 (6318) :518-520
[9]   THE FOLDING OF AN ENZYME .1. THEORY OF PROTEIN ENGINEERING ANALYSIS OF STABILITY AND PATHWAY OF PROTEIN FOLDING [J].
FERSHT, AR ;
MATOUSCHEK, A ;
SERRANO, L .
JOURNAL OF MOLECULAR BIOLOGY, 1992, 224 (03) :771-782
[10]   STRUCTURAL ENERGETICS OF THE MOLTEN GLOBULE STATE [J].
HAYNIE, DT ;
FREIRE, E .
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 1993, 16 (02) :115-140
123