IN-SITU HYBRIDIZATION AND NORTHERN BLOT DEMONSTRATION OF HLA-G MESSENGER-RNA IN HUMAN TROPHOBLAST POPULATIONS BY LOCUS-SPECIFIC OLIGONUCLEOTIDE

Trophoblast cells from normal first trimester pregnancies have been shown to express the non-classical class I molecule, HLA-G, which is nonpolymorphic and has a heavy chain of 40 kD. In this study, in situ hybridization experiments were performed using a biotinylated HLA-G specific oligonucleotide on sections of normal placenta including the implantation site. HLA-G mRNA was identified in all extravillous trophoblast populations including the cytotrophoblast cell columns, interstitial trophoblast, endovascular trophoblast, and placental bed giant cells. In addition, villous cytotrophoblast and villous mesenchymal cells also contained HLA-G transcripts, but villous syncytiotrophoblast was always negative.