A SIMPLE AND ECONOMICAL STRATEGY FOR DOWNSTREAM PROCESSING OF SPECIFIC ANTIBODIES TO HUMAN TRANSFERRIN FROM EGG-YOLK

被引：30
作者：
NTAKARUTIMANA, V
DEMEDTS, P
VANSANDE, M
SCHARPE, S
机构：
[1] UNIV INSTELLING ANTWERP,MED BIOCHEM LAB,UNIV PLEIN 1,B-2610 WILRIJK,BELGIUM
[2] INST TROP MED,CLIN BIOL LAB,B-2000 ANTWERP 1,BELGIUM
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1992年 / 153卷 / 1-2期
关键词：
EGG-YOLK ANTIBODY; IGY; IMMUNIZED HEN; ANTIBODY PURIFICATION; AFFINITY CHROMATOGRAPHY; TRANSFERRIN; NEPHELOMETRY;
D O I：
10.1016/0022-1759(92)90315-K
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid and economical procedure for extraction of antibodies from egg yolk is described. Laying hens were immunized with human transferrin and extracts of egg-yolk were purified with a procedure based on affinity chromatography. The resulting purified antibodies were evaluated in a nephelometric system for the assay of transferrin in human sera. The results agreed closely with those obtained with a commercially available anti-human transferrin serum from rabbits.
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页码：133 / 140
页数：8
相关论文
共 18 条
  • [1] RAPID METHOD OF EXTRACTION OF ANTIBODIES FROM HEN EGG-YOLK
    BADE, H
    STEGEMANN, H
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1984, 72 (02) : 421 - 426
  • [2] CARROLL SB, 1983, J BIOL CHEM, V258, P24
  • [3] THE BIOLOGY OF TRANSFERRIN
    DEJONG, G
    VANDIJK, JP
    VANEIJK, HG
    [J]. CLINICA CHIMICA ACTA, 1990, 190 (1-2) : 1 - 46
  • [4] FEINBERG JG, 1957, INT ARCH ALLER A IMM, V11, P127
  • [5] EFFICIENT PRODUCTION OF CHICKEN EGG-YOLK ANTIBODIES AGAINST A CONSERVED MAMMALIAN PROTEIN
    GASSMANN, M
    THOMMES, P
    WEISER, T
    HUBSCHER, U
    [J]. FASEB JOURNAL, 1990, 4 (08) : 2528 - 2532
  • [6] HARBOE N, 1973, Scandinavian Journal of Immunology, V2, P161
  • [7] PURIFICATION OF EGG-YOLK IMMUNOGLOBULINS - A 2-STEP PROCEDURE USING HYDROPHOBIC INTERACTION CHROMATOGRAPHY AND GEL-FILTRATION
    HASSL, A
    ASPOCK, H
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1988, 110 (02) : 225 - 228
  • [8] EGGS - CONVENIENTLY PACKAGED ANTIBODIES - METHODS FOR PURIFICATION OF YOLK IGG
    JENSENIUS, JC
    ANDERSEN, I
    HAU, J
    CRONE, M
    KOCH, C
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1981, 46 (01) : 63 - 68
  • [9] KINT A, 1987, ARCH INT PHYS BIOCH, V92, pB215
  • [10] LARSSON A, 1991, CLIN CHEM, V37, P411
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