CLONING AND EXPRESSION OF CDNA-ENCODING HUMAN BETACELLULIN, A NEW MEMBER OF THE EGF FAMILY

被引：114

作者：

SASADA, R ^{[1
]}

ONO, Y ^{[1
]}

TANIYAMA, Y ^{[1
]}

SHING, Y ^{[1
]}

FOLKMAN, J ^{[1
]}

IGARASHI, K ^{[1
]}

机构：

[1] HARVARD UNIV,CHILDRENS HOSP,SCH MED,DEPT SURG,BOSTON,MA 02115

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 190卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1173

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Betacellulin (BTC) is a novel growth factor purified from the conditioned media of mouse pancreatic beta tumor cells and has been found to be a new member of the epidermal growth factor (EGF) family. The cDNA encoding human BTC has been cloned from a cDNA library prepared from human breast adenocarcinoma cell line MCF-7. The nucleotide sequence encodes a polypeptide which consists of 178 amino acid residues including a putative signal sequence, indicating that the structural organization of human BTC is similar to that of mouse BTC. The amino acid sequence of the human BTC precursor protein exhibits 79% similarity with that of the mouse precursor protein. The BTC gene was found to be expressed in several mouse tissues including kidney and liver as well as in a mouse beta tumor cell line and MCF-7 cells, suggesting that BTC might play a physiological role in normal tissues. © 1993 Academic Press, Inc.

引用

页码：1173 / 1179

页数：7

共 21 条

[1]

BELL GI, 1986, NUCLEIC ACIDS RES, V14, P8426

[2]

Carpenter G., 1990, HDB EXPT PHARM, P69

[3] HUMAN TRANSFORMING GROWTH FACTOR-ALPHA - PRECURSOR STRUCTURE AND EXPRESSION IN ESCHERICHIA-COLI [J].

DERYNCK, R ;

ROBERTS, AB ;

WINKLER, ME ;

CHEN, EY ;

GOEDDEL, DV .

CELL, 1984, 38 (01) :287-297

[4] THE PHYSIOLOGY OF TRANSFORMING GROWTH-FACTOR-ALPHA [J].

DERYNCK, R .

ADVANCES IN CANCER RESEARCH, 1992, 58 :27-52

[5] INDUCTION OF ANGIOGENESIS DURING THE TRANSITION FROM HYPERPLASIA TO NEOPLASIA [J].

FOLKMAN, J ;

WATSON, K ;

INGBER, D ;

HANAHAN, D .

NATURE, 1989, 339 (6219) :58-61

[6] SV40-TRANSFORMED SIMIAN CELLS SUPPORT THE REPLICATION OF EARLY SV40 MUTANTS [J].

GLUZMAN, Y .

CELL, 1981, 23 (01) :175-182

[7] TRANSFORMATION OF RAT CELLS BY DNA OF HUMAN ADENOVIRUS-5 [J].

GRAHAM, FL ;

VANDEREB, AJ .

VIROLOGY, 1973, 54 (02) :536-539

[8] HERITABLE FORMATION OF PANCREATIC BETA-CELL TUMORS IN TRANSGENIC MICE EXPRESSING RECOMBINANT INSULIN SIMIAN VIRUS-40 ONCOGENES [J].

HANAHAN, D .

NATURE, 1985, 315 (6015) :115-122

[9] A HEPARIN-BINDING GROWTH-FACTOR SECRETED BY MACROPHAGE-LIKE CELLS THAT IS RELATED TO EGF [J].

HIGASHIYAMA, S ;

ABRAHAM, JA ;

MILLER, J ;

FIDDES, JC ;

KLAGSBRUN, M .

SCIENCE, 1991, 251 (4996) :936-939

[10] QUANTITATIVE SYSTEM FOR ASSAY OF MALIGNANT TRANSFORMATION BY CHEMICAL CARCINOGENS USING A CLONE DERIVED FROM BALB/3T3 [J].

KAKUNAGA, T .

INTERNATIONAL JOURNAL OF CANCER, 1973, 12 (02) :463-473

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